摘要
Rumen content-associated (RC) and epithelial tissue-attached (RT) bacterial communities are composed of different phylotypes and play distinctive roles. This study aimed to compare the composition of the RT and RC bacterial communities of steers differing in feed efficiency. The microbiota of RT and RC samples collected from sixteen beef steers with high or low residual feed intake (RFI) were analyzed through sequencing of partial 16S rRNA gene amplicons. Bacteroidetes, Proteobacteria and Firmicutes were the predominant phyla and Prevotella was the most abundant genus in both RC and RT bacterial communities. In total, 19 OTUs of the RC samples and 19 OTUs of the RT samples were differentially abundant (DA) between H-RFI and l-RFI steers. Among them, a common DA OTU belonged to Prevotella genus was identified in both RC and RT samples, making it the potential key microbial marker for indicating feed efficiency of steers. The co-occurrence of the DA OTUs among RT and RC samples suggest the importance of these two communities function as a complete system in influencing host feed efficiency.
摘要译文
瘤胃含量相关（RC）和上皮组织附着（RT）细菌群落由不同的文学型组成并发挥独特的作用。本研究旨在比较饲料效率不同的带状器的RT和RC细菌群落的组成。通过部分16S rRNA基因扩增子测序分析从具有高或低残余进料摄入量（RFI）的16次牛肉阉牛（RFI）的RT和RC样品的微生物。菌斑，植物体外和迫切性是主要的植物和PREVOTALLA是RC和RT细菌社区中最丰富的属。总共，RC样品的19个OTU和RT样品的19个OTU在H-RFI和L-RFI操纵子之间差异丰富（DA）。其中，在RC和RT样品中鉴定了属于PREVOTELLA属的常见的DA OTU，使其成为指示阉牛饲料效率的潜在关键微生物标记。 RT和RC样本中DA OTU的共同发生表明这两个社区函数作为影响主机馈送效率的完整系统的重要性。
Mi Zhou[a];Bibaswan Ghoshal[b];Paul Stothard[a];Le Luo Guan[a]. Distinctive roles between rumen epimural and content bacterial communities on beef cattle feed efficiency: A combined analysis[J]. Current Research in Microbial Sciences, 2021,2