摘要
Different dietary and feed additive strategies have been developed to reduce the liver abscess in feedlot cattle, but liver abscesses are still a major problem in beef production. We have limited knowledge about how rumen microbial communities interact with host epithelial gene expression in healthy and liver-abscessed animals. The objective of this study was to investigate the associations between the rumen content associated and rumen epimural microbiome and epithelial gene expression in liver-abscessed and healthy animals. To this end, we collected the ruminal contents and tissue samples from healthy (N=30; score=0, steers n=19 and heifers n=11) and liver-abscessed (N=30; score=A+, steers n=21 and heifers n=9) feedlot cattle at harvest. The bacterial community compositions in the ruminal contents and papillae were evaluated via 16S rDNA sequencing of the V4 region using the Illumina MiSeq platform. Additionally, total RNA was extracted from rumen epithelial tissues and sequenced using the Illumina NextSeq platform. The permutational analysis (PERMANOVA) on Bray Curtis distances matrices showed the microbial community in the ruminal contents was significantly different (P< 0.001) from the bacterial community observed in rumen papillae. The ruminal contents contained a higher abundance of Bacteroidetes and Proteobacteria while papillae contained higher abundance of Firmicutes. The epimural microbiota was different (P< 0.01) between healthy and liver abscessed animals while ruminal contents microbiome was not different between the two groups. The DeSeq2 algorithm identified differentially expressed genes (221) related to MAPK, NF-kappa B signaling pathway, immune and inflammatory response in liver-abscessed animals. Additionally, a wide range of epimural bacterial taxa were correlated (-0.52 to 0.67) with differentially expressed genes. These data demonstrate the interaction between epimural microbiota and the host and its effect on liver abscesses, and indicate the need to study the epimural microbiome for its impact on liver abscesses in feedlot cattle. USDA is an equal opportunity provider and employer.
摘要译文
已经开发出不同的饮食和饲料添加剂策略来减少饲养场牛的肝脓肿，但肝脓肿仍然是牛肉生产中的主要问题。我们对瘤胃微生物群落如何与健康和肝脏抗肿瘤动物中的宿主上皮基因表达相互作用有限。这项研究的目的是研究肝脏和健康动物中相关的瘤胃含量与瘤胃表达微生物组和上皮基因表达之间的关联。为此，我们从健康中收集了瘤胃含量和组织样品（n = 30;得分= 0，转向n = 19和heifers n = 11）和肝脏 - abscess（n = 30;得分= a+，n = 21和小母牛n = 9）收获时饲养牛。使用Illumina Miseq平台通过V4区域的16S rDNA测序评估了瘤胃含量和乳头状的细菌群落组成。此外，从瘤胃上皮组织中提取总RNA，并使用Illumina NextSeq平台进行测序。 Bray Curtis距离矩阵上的置换分析（Permanova）表明，瘤胃中的微生物群落与在瘤胃乳头状乳突中观察到的细菌群落明显不同（P <0.001）。瘤胃含量含有较高的细菌植物和蛋白质，而乳头状含有较高的富公司。健康和肝脏脓肿的动物之间的表皮微生物群不同（p <0.01），而两组之间的瘤胃含量微生物组没有差异。 DESEQ2算法鉴定出与MAPK，NF-KAPPA B信号通路，免疫和炎症反应有关的差异表达的基因（221）。此外，将广泛的表皮细菌分类群与差异表达的基因相关（-0.52至0.67）。这些数据证明了表皮微生物群与宿主之间的相互作用及其对肝脓肿的影响，并表明需要研究表皮微生物组对饲料牛中肝脓肿的影响。美国农业部是机会均等的提供者和雇主。
Waseem Abbas; Am;a K Lindholm-Perry; Brittney N Keel; James E Wells; Kristin Hales; Allison Knoell; Henry A Paz; ; Samodha Fern;o. 224 Epimural microbiota and rumen epithelial gene expression in healthy and liver-abscessed animals[J]. Journal of Animal Science, 2020,98(Sup3)