期刊文献

Rumen Epithelial Communities Share a Core Bacterial Microbiota: A Meta-Analysis of 16S rRNA Gene Illumina MiSeq Sequencing Datasets 收藏

瘤胃上皮社区共享核心细菌微生物：16S rRNA基因Illumina Miseq测序数据集的Meta分析
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作者
Chiron J. ;erson; Lucas R. Koester; ; Stephan Schmitz-Esser
作者单位
[1]Department of Animal Science, Iowa State University, Ames, IA, United States[2]Interdepartmental Microbiology Graduate Program, Iowa State University, Ames, IA, United StatesFind articles by Chiron J. Anderson[2]Interdepartmental Microbiology Graduate Program, Iowa State University, Ames, IA, United States[3]Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA, United StatesFind articles by Lucas R. Koester[1]Department of Animal Scien
关键词
rumen wall, rumen epithelium, epimural, microbiota, ruminant
关键词译文
瘤胃墙，瘤胃上皮，互联网，微生物群，反刍动物
发布日期
2021
DOI
10.3389/fmicb.2021.625400
来源信息
Frontiers in Microbiology ISSN：1664-302X, 2021年, 12卷
摘要
In this meta-analysis, 17 rumen epithelial 16S rRNA gene Illumina MiSeq amplicon sequencing data sets were analyzed to identify a core rumen epithelial microbiota and core rumen epithelial OTUs shared between the different studies included. Sequences were quality-filtered and screened for chimeric sequences before performing closed-reference 97% OTU clustering, and de novo 97% OTU clustering. Closed-reference OTU clustering identified the core rumen epithelial OTUs, defined as any OTU present in
摘要译文
在该荟萃分析中，分析了17个瘤胃上皮16S rRNA基因Illumina Miseq扩增子测序数据集以鉴定包括不同研究的核心瘤胃上皮微生物和核心瘤胃上皮OTU。在进行闭合参考97％OTU聚类之前，序列被质量过滤并筛选嵌合序列，以及De Novo 97％OTU聚类。闭合参考OTU聚类识别核心瘤胃上皮OTU，定义为出现的任何OTU
Chiron J. ;erson; Lucas R. Koester; ; Stephan Schmitz-Esser. Rumen Epithelial Communities Share a Core Bacterial Microbiota: A Meta-Analysis of 16S rRNA Gene Illumina MiSeq Sequencing Datasets[J]. Frontiers in Microbiology, 2021,12