摘要
The connections between taste receptor cells (TRCs) and innervating gustatory neurons are formed in a mutually dependent manner during development. To investigate whether a change in the ratio of cell types that compose taste buds influences the number of innervating gustatory neurons, we analyzed the proportion of gustatory neurons that transmit sour taste signals in adult Skn-1a−/− mice in which the number of sour TRCs is greatly increased. We generated polycystic kidney disease 1 like 3-wheat germ agglutinin (pkd1l3-WGA)/Skn-1a+/+ and pkd1l3-WGA/Skn-1a−/− mice by crossing Skn-1a−/− mice and pkd1l3-WGA transgenic mice, in which neural pathways of sour taste signals can be visualized. The number of WGA-positive cells in the circumvallate papillae is 3-fold higher in taste buds of pkd1l3-WGA/Skn-1a−/− mice relative to pkd1l3-WGA/Skn-1a+/+ mice. Intriguingly, the ratio of WGA-positive neurons to P2X2-expressing gustatory neurons in nodose/petrosal ganglia was similar between pkd1l3-WGA/Skn-1a+/+ and pkd1l3-WGA/Skn-1a−/− mice. In conclusion, an alteration in the ratio of cell types that compose taste buds does not influence the number of gustatory neurons that transmit sour taste signals.
摘要译文
在开发期间，味觉感受器细胞（TRC）和神经滋养神经元之间的联系以相互依赖的方式形成为了调查构成味蕾的细胞类型比例的变化是否影响了神经滋养神经元的数量，我们分析了在成年Skn-1a sup鈭 鈭 / sup小鼠中传播酸味信号的味觉神经元的比例，其中酸性TRCs的数量大大增加多囊肾病1例如3-小麦胚芽凝集素（pkd113-WGA）/ Skn-1a sup + / + / sup和pkd113-WGA / Skn-1a / sup小鼠Skn-1a小鼠和pkd113-WGA转基因小鼠，其中酸味信号的神经通路可以被可视化相对于pkd113-WGA / Skn-1a，在圆形乳头中的WGA阳性细胞的数量比pkd113-WGA / Skn-1a / u / u p + / + / sup小鼠有趣的是，在pkd1l3-WGA / Skn-1a / sup + / +和pkd113-WGA / Skn-1a之间，WGA阳性神经元与P2X 2表达的嗜酸性神经元在结节/石蜡神经节中的比例相似鈭 鈭 / sup小鼠总之，构成味蕾的细胞类型比例的变化不影响传播酸味信号的味觉神经元数量
Naohiro Maeda[a][1]; Masataka Narukawa[a][1]; Yoshiro Ishimaru[a][b]; Kurumi Yamamoto[a]; Takumi Misaka[a]; Keiko Abe[a][c]. A large increase of sour taste receptor cells in Skn-1-deficient mice does not alter the number of their sour taste signal-transmitting gustatory neurons[J]. Neuroscience Letters, 2017,648: 53–58