摘要
n-Butanol is a renewable resource with a wide range of applications. Its physicochemical properties make it a potential substitute for gasoline. Saccharomyces cerevisiae can produce n-butanol via amino acid catabolic pathways, but the use of pure amino acids is economically unfeasible for large-scale production. The aim of this study was to optimize the production of n-butanol by S. cerevisiae from protein-rich agro-industrial by-products (sunflower and poultry offal meals). By-products were characterized according to their total protein and free amino acid contents and subjected to enzymatic hydrolysis. Protein hydrolysates were used as nitrogen sources for the production of n-butanol by S. cerevisiae, but only poultry offal meal hydrolysate (POMH) afforded detectable levels of n-butanol. Under optimized conditions (carbon/nitrogen ratio of 2 and working volume of 60%), 59.94 mg/L of n-butanol was produced using POMH and glucose as substrates. The low-cost agro-industrial by-product showed great potential to be used in the production of n-butanol by S. cerevisiae. Other protein-rich residues may also find application in biofuel production by yeasts.
摘要译文
正丁醇是一种可再生资源，具有广泛的应用范围。其理化性质使其成为汽油的潜在替代品。酿酒酵母可以通过氨基酸分解代谢途径生产正丁醇，但是使用纯氨基酸在经济上不适合大规模生产。这项研究的目的是优化酿酒酵母从富含蛋白质的农业工业副产品（向日葵和家禽内脏粉）中生产正丁醇的方法。根据副产物的总蛋白质和游离氨基酸含量对其进行表征，并进行酶水解。蛋白质水解物被用作酿酒酵母生产正丁醇的氮源，但是只有禽畜内脏粉水解物（POMH）提供了可检测水平的正丁醇。在最佳条件下（碳氮比为2，工作体积为60％），以POMH和葡萄糖为底物可生产59.94 mg / L的正丁醇。低成本的农业工业副产品显示出巨大的潜力，可用于酿酒酵母生产正丁醇。其他富含蛋白质的残留物也可能在酵母生物燃料生产中得到应用。
Bruno A. S. Santos[1];Suéllen P. H. Azambuja[1];Patrícia F. ávila[1];Maria Teresa B. Pacheco[2];Rosana Goldbeck[1]. n -Butanol production by Saccharomyces cerevisiae from protein-rich agro-industrial by-products[J]. Brazilian Journal of Microbiology, 2020,51(4): 1655-1664