摘要
The growing food adulteration and increasing antibiotic intake has led to deterioration of gut health in humans. Probiotics are feed supplements in the form of live microorganisms that have a positive impact on the host by improving intestinal microbial balance. They offer a variety of important and documented beneficial effects, including the prevention of various disorders and modulating the immune response. Lactic acid bacteria (LAB) are class of organisms possessing probiotic potential and hence, can be used for producing novel therapeutics for tackling the above-mentioned health concerns. This work focuses on isolation and exploration of probiotic potential of LAB which can confer health benefits when consumed in an adequate manner. A total of 48 bacteria were isolated on the Lactobacillus selection medium (LSB) from fourteen different food sources such as milk, milk-based products, and non-dairy fermented foods. The isolates were assessed for tolerance against various physiochemical conditions, antibiofilm activity, antimicrobial activity, antibiotic resistance, auto and co-aggregation, and hemolytic activity. Six potential LAB isolates exhibited tolerance against a higher concentration of bile acid, NaCl, phenol and stimulated gastric juice. The isolates were negative for gelatinase and hemolytic activity. All the six isolates showed antimicrobial activity against the test organisms, and antimicrobial resistance against the selected antibiotics. In addition to this, all LAB isolates indicated co-aggregation with test pathogens and adhesion to silicone oil and paraffin oil, respectively. Further, these isolates were found to be biofilm producers and exhibited anti-biofilm activity. The Lactic acid bacteria were found to fulfill the basic requirements of a probiotic bacteria and hence, can be used for human consumption resulting in various health benefits.
摘要译文
不断增长的食物掺假和抗生素摄入量增加导致人类肠道健康的恶化。益生菌是活微生物的形式的饲料补充剂，通过改善肠道微生物平衡对宿主产生积极影响。它们提供了各种重要和有记录的有益作用，包括预防各种疾病并调节免疫反应。乳酸细菌（LAB）是具有益生菌潜力的生物类别，因此可用于生产新型的治疗剂来解决上述健康问题。这项工作的重点是隔离和探索实验室的益生菌潜力，这些益生菌的潜力可以在充分的方式消费时赋予健康益处。从十四种不同的食物来源（例如牛奶，基于牛奶的产品和非乳制品发酵食品）的乳酸杆菌选择培养基（LSB）上分离出48种细菌。评估了分离株的耐受性，以抗各种生理化学条件，抗生素活性，抗菌活性，抗生素耐药性，自动和聚集活性以及溶血活性。六个潜在的实验室分离株表现出对较高浓度的胆汁酸，NaCl，苯酚和刺激胃汁的耐受性。分离株对于明胶酶和溶血活性为阴性。所有六种分离株均显示针对测试生物的抗菌活性，以及对所选抗生素的抗菌素耐药性。除此之外，所有实验室分离株均表明分别与测试病原体和硅油和石蜡油的粘附共同聚集。此外，发现这些分离株是生物膜生产者，并表现出抗生物膜活性。发现乳酸细菌可满足益生菌细菌的基本要求，因此可用于人类食用，从而产生各种健康益处。
Vikas Jha;Chinmayi Sarang;Dattatray Sawant;Kavita Nalawade;Vrushali Dhamapurkar;Navdeep Kaur;Kabir Thakur;Shruti Amin;Pratyusha Mane;Aishwarya Marath. Exploration of Probiotic Potential of Lactic Acid Bacteria Isolated from Different Food Sources[J]. American Journal of BioScience, 2022,10(3): 118-130