摘要
The present study investigated the probiotic properties of lactic acid bacteria (LAB) isolated from traditionally produced sauerkraut. For this reason, total of thirty-nine isolates were obtained from sauerkraut produced at homes by traditional methods around Izmir city. Development of different pH, temperature and salt values, tolerance to bile salt, resistance to 0.4% (v/v) phenol, development in the presence of pepsin and pancreatin, antimicrobial activity, resistance to antibiotics, hemolytic activity and β-Galactosidase activity analyzes were applied to the isolates for determining probiotic properties. Within the scope of the study, except for four isolates (HL7, HL13, HL22 and HL31), which were found to be resistant to all antibiotics, all other isolates could be considered as probiotics. However, among these isolates, the probiotic potential of GL1, GL3, GL13, HL8, HL9, HL10, HL11, HL12, HL14 , HL18, HL25, HL26, HL28, HL29, HL33 was found higher than other isolates when investigated in terms of growth at low pH and high NaCl concentration, growth in the presence of bile salt, phenol, pepsin and pancreatin, broad antimicrobial spectrum, high antimicrobial effect, sensitivity to antibiotics and β-galactosidase activity. The results showed that sauerkraut is an important probiotic source.
摘要译文
本研究研究了从传统产生的酸菜中分离出的乳酸菌（LAB）的益生菌特性。因此，从伊兹米尔市（Izmir City）周围的传统方法在房屋中生产的酸菜中获得了总共39个分离株。开发不同的pH值，温度和盐值，对胆汁盐的耐受性，对0.4％（v/v）苯酚的耐药性，在存在胃蛋白酶和胰素的存在下发育，抗菌活性，抗抗生素的耐药性，溶血活性和β-半乳糖苷酶活性分析将其应用于分离株以确定益生菌特性。在研究范围内，发现四种分离株（HL7，HL13，HL22和HL31）被发现对所有抗生素具有抗性，所有其他分离株都可以视为益生菌。然而，在这些分离株中，GL1，GL3，GL13，HL8，HL9，HL9，HL11，HL11，HL12，HL12，HL14，HL18，HL18，HL25，HL26，HL26，HL28，HL28，HL29，HL29，HL33，HL33被发现比其他分离株高于其他分离物，当在其他隔离率高于其他分离物时，当在低pH值和高NaCl浓度下的生长，存在胆汁盐，苯酚，胃蛋白酶和胰腺素的生长，广泛的抗菌谱，高抗菌作用，对抗生素的敏感性和β-半乳糖苷酶活性。结果表明，酸菜是重要的益生菌来源。