期刊文献

Wine Grape Pomace as a Dietary Supplement to Improve Semen Quality in Boars 收藏

葡萄酒葡萄Pomace作为饮食补充剂，以提高公猪的精液质量

原文求助发布源

作者

Armando Quintero‐Moreno (https://orcid.org/0000-0002-8998-6499) [1];Cristina Martínez‐López [2];Chiara Luongo [3];María Luz García [4];María José Argente [5];Gema Romero [6];Cristina Pérez‐Patiño [7];José Ramón Díaz [8];Francisco A. García‐Vázquez [9];Pedro José Llamas‐López [10];

作者单位

[1]Departamento de Fisiología, Facultad de Veterinaria, Campus de Excelencia Mare Nostrum Universidad de Murcia Murcia Spain; [2]Departamento de Fisiología, Facultad de Veterinaria, Campus de Excelencia Mare Nostrum Universidad de Murcia Murcia Spain; [3]Departamento de Fisiología, Facultad de Veterinaria, Campus de Excelencia Mare Nostrum Universidad de Murcia Murcia Spain; [4]Instituto de Investigación e Investigación Agroalimentaria y Agroambiental CIAGRO‐UMH Orihuela Spain; [5]Instituto de Investigación e Investigación Agroalimentaria y Agroambiental CIAGRO‐UMH Orihuela Spain; [6]Instituto de Investigación e Investigación Agroalimentaria y Agroambiental CIAGRO‐UMH Orihuela Spain; [7]CEFU S.A. Murcia Spain; [8]Instituto de Investigación e Investigación Agroalimentaria y Agroambiental CIAGRO‐UMH Orihuela Spain; [9]Departamento de Fisiología, Facultad de Veterinaria, Campus de Excelencia Mare Nostrum Universidad de Murcia Murcia Spain; [10]Instituto de Investigación e Investigación Agroalimentaria y Agroambiental CIAGRO‐UMH Orihuela Spain;

发布日期

2025-4-7

DOI

10.1111/rda.70049

来源信息

Reproduction in Domestic Animals ISSN：0936-6768, 2025年, 60卷, 4期

摘要

【ABSTRACT】Boar spermatozoa are highly susceptible to oxidative damage due to their high content of unsaturated fatty acids, which are prone to disruption by reactive oxygen species (ROS). Excessive ROS can induce lipid peroxidation, DNA fragmentation and impaired enzyme activity, ultimately reducing sperm quality and reproductive performance. Wine grape pomace (WGP), a by‐product of the winemaking process, is rich in polyphenols, including flavonoids (anthocyanins and quercetin), stilbenes (resveratrol) and tannins, which possess strong antioxidant properties. This study aimed to evaluate the effects of dietary supplementation with 4% WGP on boar ejaculate output and sperm quality during storage. Twenty boars were divided into two groups: a control group fed a standard diet and a WGP group supplemented with 4% WGP for 4 months. Semen samples were collected and analysed for ejaculate number of sperm per ml, total antioxidant capacity (in seminal plasma) and quality parameters (motility, kinematic parameters, mitochondrial activity, acrosome integrity, viability) after 1, 3 and 5 days of refrigerated storage. Results showed that WGP supplementation increased the number of sperm per ml compared to the control group (p < 0.05), resulting in approximately two additional seminal doses per ejaculate, without negatively affecting other seminal parameters (p > 0.05) and refrigeration storage (p > 0.05). This improvement in sperm concentration could enhance the profitability of swine semen production by increasing the number of doses produced per boar annually. Given the low cost of WGP (10.03 €/boar/year), this strategy could offer a cost‐effective approach to improving reproductive performance in boars. These findings support further research into optimising WGP inclusion levels and exploring its broader impacts on boar fertility and reproductive efficiency.

摘要译文

【摘要】野猪精子高度易受氧化损伤，因为它们的不饱和脂肪酸含量高，这很容易被活性氧（ROS）破坏。过多的ROS会诱导脂质过氧化，DNA碎片和酶活性受损，最终降低精子质量和生殖性能。葡萄酒葡萄Pomace（WGP）是酿酒过程的副产品，富含多酚，包括类黄酮（花青素和槲皮素），Stilbenes（白藜芦醇）和单宁，具有强大的抗氧化特性。这项研究旨在评估4％WGP饮食补充对储存过程中饮食的影响。将20只公猪分为两组：一个喂养标准饮食的对照组和一个补充4％WGP的WGP组4个月。收集精液样品并分析每ML的射精数量，总抗氧化能力（以精确血浆）和质量参数（运动性，运动学参数，线粒体活性，种田完整性，活性，活性，活力），1、3和5天后1、3和5天后。结果表明，与对照组相比，补充WGP每ML的精子数量增加（P <0.05），导致每射射击大约另外两次精确剂量，而不会对其他精确参数产生负面影响（P> 0.05）和冷藏储存（P> 0.05）。精子浓度的这种改善可以通过每年增加每头公猪的剂量数量来提高猪精液产量的盈利能力。鉴于WGP的低成本（10.03欧元/年/年），该策略可以提供一种具有成本效益的方法来改善公猪的生殖性能。这些发现支持进一步的研究，以优化WGP纳入水平，并探索其对公猪的生育和生殖效率的更广泛影响。