摘要
Enterococcus spp. are normal intestinal tract microflorae found in poultry. However, the last decades have shown that several species, e.g., Enterococcus cecorum, have become emerging pathogens in broilers and may cause numerous losses in flocks. In this study, two combinations (H1 and H2) of menthol, 1,8-cineol, linalool, methyl salicylate, γ-terpinene, p-cymene, trans-anethole, terpinen-4-ol and thymol were used in an in vitro model, analyzing its effectiveness against the strains E. cecorum, E. faecalis, E. faecium, E. hirae and E. gallinarum isolated from broiler chickens from industrial farms. To identify the isolated strains classical microbiological methods and VITEK 2 GP cards were used. Moreover for E. cecorum a PCR test was used.. Antibiotic sensitivity (MIC) tests were performed for all the strains. For the composition H1, the effective dilution for E. cecorum and E. hirae strains was 1:512, and for E. faecalis, E. faecium and E. gallinarum, 1:1024. The second mixture (H2) showed very similar results with an effectiveness at 1:512 for E. cecorum and E. hirae and 1:1024 for E. faecalis, E. faecium and E. gallinarum. The presented results suggest that the proposed composition is effective against selected strains of Enterococcus in an in vitro model, and its effect is comparable to classical antibiotics used to treat this pathogen in poultry. This may suggest that this product may also be effective in vivo and provide effective support in the management of enterococcosis in broiler chickens.
摘要译文
肠球菌属。是在家禽中发现的正常肠道微生物。然而，过去几十年表明，例如肉鸡中的几种物种，例如肠球菌已成为新兴的病原体，并可能在羊群中造成许多损失。在这项研究中，薄荷酚，1,8-甲醇，林纳尔，水杨酸甲基甲酸，γ-苯二烯，p-甲苯，跨氨基甲烷，Terpinen-4-ol和胸腺的两种组合（H1和H2）用于体内模型，分析了其针对菌株的有效性。E。切口，E。Faecalis，E。粪肠球菌，Hirae E. hirae和E. gallinarum从工业农场分离出来的肉鸡。为了识别孤立的菌株，使用了经典微生物方法和Vitek 2 GP卡。此外，对于大肠杆菌，都使用了PCR测试。所有菌株均进行了抗生素灵敏度（MIC）测试。对于组成H1，对切口大肠杆菌和hirae菌株的有效稀释为1：512，而粪肠球菌，大肠杆菌和大肠杆菌（E. gallinarum），1：1024。第二种混合物（H2）显示出非常相似的结果，剖腹和hirae的有效性在1：512，而粪肠球菌，粪肠球菌和Gallinarum的E. faecalis和1：1024。提出的结果表明，所提出的组合物在体外模型中对选定的肠球菌菌株有效，并且其作用与用于治疗该病原体在家禽中这种病原体的经典抗生素相当。这可能表明该产品在体内也可能有效，并为肉鸡肠球菌病的管理提供有效的支持。
Karolina Wódz (0000-0002-9605-6409) [1];Karolina A. Chodkowska (0000-0001-6839-0824) [2];Hubert Iwiński [3];Henryk Różański [4];Jakub Wojciechowski [5];. In Vitro Evaluation of Phytobiotic Mixture Antibacterial Potential against Enterococcus spp. Strains Isolated from Broiler Chicken[J]. International Journal of Molecular Sciences, 2024,25(9): 4797