期刊文献

Antioxidant and Anti-Inflammatory Activity of a New Formulation of Slow-Release Amino Acids in Human Intestinal Caco-2 Cells 收藏

人类肠道Caco-2细胞中缓慢释放氨基酸的新配方的抗氧化剂和抗炎活性

原文求助发布源

作者

Carlotta Bollati (https://orcid.org/0000-0002-5698-1325) [1];Martina Tosi (https://orcid.org/0000-0003-4859-5288) [2];Lorenza d’Adduzio (https://orcid.org/0000-0002-3356-0595) [3];Melissa Fanzaga (https://orcid.org/0009-0002-3175-924X) [4];Alberto Burlina (https://orcid.org/0000-0001-7724-137X) [5];Gianvincenzo Zuccotti (https://orcid.org/0000-0002-2795-9874) [6];Carmen Lammi (https://orcid.org/0000-0002-7428-4486) [7];Elvira Verduci (https://orcid.org/0000-0003-2111-3111) [8];

作者单位

[1]Department of Pharmaceutical Sciences, University of Milan, 20133 Milan, Italy; [2]Department of Pediatrics, Vittore Buzzi Children’s Hospital, University of Milan, 20154 Milan, Italy; [3]Department of Pharmaceutical Sciences, University of Milan, 20133 Milan, Italy; [4]Department of Pharmaceutical Sciences, University of Milan, 20133 Milan, Italy; [5]Division of Inherited Metabolic Diseases, Department of Women’s and Children’s Health, Reference Centre Expanded Newborn Screening, University Hospital, 35128 Padua, Italy; [6]Department of Pediatrics, Vittore Buzzi Children’s Hospital, University of Milan, 20154 Milan, Italy; [7]Department of Pharmaceutical Sciences, University of Milan, 20133 Milan, Italy; [8]Department of Health Sciences, University of Milan, 20142 Milan, Italy;

发布日期

2025-2-26

页码

271

DOI

10.3390/antiox14030271

来源信息

Antioxidants ISSN：2076-3921, 2025年, 14卷, 3期, 271页

摘要

Phenylalanine (Phe)-free protein substitutes (PSs) are used to provide an adequate intake of amino acids (AAs), except Phe, allowing control of blood Phe levels in patients with Phenylketonuria (PKU). Although indicated as a standard dietary treatment for these patients, free AAs mixtures are not absorbed as natural proteins, thus creating an oxidized and inflamed state in the intestine. Nowadays, PSs on the market also include slow-release amino acids (SR-AAs) formulas. The present work aims to investigate the effects of an SR-AAs formula on both oxidative and inflammatory status in human intestinal Caco-2 cells, comparing its mechanism of action with that of a mixture of free AAs. In more detail, oxidative stress and inflammation were induced at the cellular level using H2O2 and lipopolysaccharides (LPSs), respectively, and both free AAs and SR-AAs PSs were tested to evaluate if they were able to restore a more balanced condition. According to our findings, free AAs aggravate the intestinal oxidative and inflammatory status caused by H2O2 and LPS in human intestinal Caco-2 cells, which SR-AAs significantly improve. In conclusion, our results offer preclinical novelty on these products’ mechanisms of action, thus improving the dietary management of patients with PKU.

摘要译文

苯丙氨酸（PHE） - 无蛋白质替代品（PSS）用于提供足够的氨基酸（AAS）摄入量，除了PHE，允许控制苯基酮（PKU）患者的血液PHE水平。尽管对这些患者的标准饮食治疗表示，但自由AAS混合物并未吸收为天然蛋白质，因此在肠中产生了氧化和发炎的状态。如今，市场上的PSS还包括缓释氨基酸（SR-AAS）配方。目前的工作旨在研究人类肠道CACO-2细胞中SR-AAS公式对氧化和炎症状态的影响，将其作用机理与自由AA的混合物进行比较。更详细地，分别使用H2O2和脂多糖（LPS）在细胞水平上诱导氧化应激和炎症，并测试了自由AAS和SR-AAS PSS，以评估他们是否能够恢复更平衡的状况。根据我们的发现，自由AAS加剧了人类肠道CACO-2细胞中H2O2和LPS引起的肠道氧化和炎症状态，SR-AAS显着改善。总之，我们的结果为这些产品的作用机理提供了临床前的新颖性，从而改善了PKU患者的饮食管理。