摘要
In this study, we utilised palm kernel cake as a substrate and fermented it with a composite of bacteria (Pediococcus pentosaceus CGMCC No. 27203 and Lactobacillus plantarum CGMCC No. 27202) and enzymes. We conducted a trial with twenty-four cattle, randomly divided into two groups of twelve cattle each. The control group (CON) was fed the standard farm diet, whereas the treatment group (PKC) received a diet with 3% of soyabean replaced by fermented palm kernel cake. The trial lasted for six weeks. The results showed no significant differences in growth performance between the PKC and CON groups. The abundance of Firmicutes, Bacteroidetes, Proteobacteria, and Spirochaetes was significantly higher in the PKC group than in the CON group. At the genus level, the abundances of Anaeroplasma, norank_f__Bacteroidales_UCG-001, norank_f__Absconditabacteriales_SR1, norank_f__p-251-o5, Prevotellaceae_NK3B31_group, Prevotellaceae_UCG-001, Prevotellaceae_UCG-004, and Treponema significantly increased in the PKC group. Lipid digestion and absorption pathways were significantly enriched in the PKC group. The results indicate that adding fermented palm kernel cake to the diet can increase the abundance of Bacteroidetes and Fibrobacteres in the rumen of beef cattle, enhancing the ability of the PKC group to degrade protein, carbohydrates, and fibrous materials in the feed, thereby improving the feed utilisation efficiency in beef cattle. Adding fermented palm kernel cake to the diet improved carbohydrate metabolism, metabolism of cofactors and vitamins, and nucleotide metabolism. Correlation analysis between the rumen microbiota and metabolic pathways showed that Prevotellaceae_UCG-001 and Prevotellaceae_UCG-003 were positively correlated with amino acid metabolism, Rikenellaceae_RC9_gut_group and Succiniclasticum were positively correlated with metabolism of cofactors and vitamins, and Prevotella and Ruminococcus were positively correlated with nucleotide metabolism. These findings elucidate the differences in rumen microbiota when fermented palm kernel cake is added to the diet, providing a theoretical basis for the application of fermented palm kernel cake in the diet of beef cattle.
摘要译文
在这项研究中，我们将棕榈仁蛋糕用作底物，并用细菌的复合材料（Pediococcus pentococcus cgmcc No. 27203和Plantarum plantarum cgmcc No. 27202）和酶进行发酵。我们对二十四头牛进行了试验，每组分为两组十二头牛。对照组（CON）喂养标准农场饮食，而治疗组（PKC）接受了饮食，其中3％的大豆被发酵棕榈仁蛋糕取代。试验持续了六个星期。结果表明，PKC和CON组之间的生长性能没有显着差异。PKC组的富公司，杆菌，蛋白质细菌和螺旋体的丰度明显高于CON组。在属水平上，厌食症的丰富性，norank_f__bacteroidales_ucg-001，norank_f__absconditabacteriales_sr1，norank_f__p-251-o5，prevotellaceae_nk3b31_group，prevotellaceae_group，prevotellaceae_ucg-001 C组。脂质消化和吸收途径在PKC组中显着富集。结果表明，将发酵的棕榈仁蛋糕添加到饮食中可以增加牛奶瘤胃中的细菌和纤维细菌的丰富性，从而增强PKC组降解蛋白质，碳水化合物和纤维材料的能力，从而改善饲料中的纤维材料肉牛的饲料利用效率。在饮食中添加发酵的棕榈仁蛋糕改善了碳水化合物的代谢，辅因子和维生素的代谢以及核苷酸代谢。瘤胃微生物群和代谢途径之间的相关分析表明，prevotellaceae_ucg-001和prevotellaceae_ucg-003与氨基酸代谢呈正相关，rikenellaceae_rc9_gut_group和Succiniclasticum与unsipertial correlist correl and cormint corfors and corfors and corfors and coroftors cofuls acctort acctort acctort acctort acctort acctort acctort acct actcort acctort acct actcort acctort acctort acctort acct actcort与核苷酸代谢相关。这些发现阐明了当将发酵的棕榈仁蛋糕添加到饮食中时，瘤胃微生物群的差异为在肉牛的饮食中应用发酵棕榈仁蛋糕提供了理论上的基础。
Wenbo Jiang; Yan Zhang; Haijian Cheng; Xin Hu; Wei You; Enliang Song; Zhiyong Hu; Fugui Jiang. Fermented Palm Kernel Cake Improves the Rumen Microbiota and Metabolome of Beef Cattle[J]. Animals, 2024,14(21): 3088