期刊文献

Effect of rumen microbiota transfaunation on the growth, rumen fermentation, and microbial community of early separated Japanese Black cattle 收藏

瘤胃微生物群转移对早期分离日本黑牛生长，瘤胃发酵和微生物群落的影响

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作者

作者单位

[1]National Agriculture and Food Research Organization Institute of Livestock and Grassland Science Tsukuba Japan; [2]National Agriculture and Food Research Organization Institute of Livestock and Grassland Science Tsukuba Japan; [3]National Livestock Breeding Center Tokachi Station Otofuke Japan; [4]National Livestock Breeding Center Tokachi Station Otofuke Japan; [5]National Livestock Breeding Center Tokachi Station Otofuke Japan; [6]National Livestock Breeding Center Tokachi Station Otofuke Japan; [7]National Livestock Breeding Center Tokachi Station Otofuke Japan; [8]National Livestock Breeding Center Tokachi Station Otofuke Japan;

发布日期

2023-10-11

DOI

10.1111/asj.13876

来源信息

Animal Science Journal ISSN：1344-3941, 2023年, 94卷, 1期

摘要

【Abstract】This study aimed to investigate the effect of rumen microbiota transfaunation on the growth, rumen fermentation, and the microbial community of Japanese Black cattle that were separated early from their dams. Here, 24 calves were separated from their dams immediately after calving, 12 of which were transfaunated via inoculation with rumen fluid from adult cattle at the age of 2 months while the remaining 12 were kept unfaunated (not‐inoculated). Feed efficiency monitoring was performed during 7–10 months of age. Body weight and feed intake were not significantly different between the transfaunated and unfaunated cattle. Transfaunation increased the relative levels of acetate and butyrate but decreased those of propionate, which increased the non‐glucogenic/glucogenic short‐chain fatty acid ratio. Microbial 16S, 18S, and ITS ribosomal RNA gene amplicon analysis showed that rumen microbial diversity and composition differed between transfaunated and unfaunated cattle; transfaunation increased the abundance of acetate‐ and butyrate‐producing bacteria, and decreased the abundance of bacterial genera associated with propionate production. Transfaunation also increased the abundance of Methanomassiliicoccaceae_group10 (1.94% vs. 0.05%) and Neocallimastix (27.1% vs. 6.8%) but decreased that of Methanomicrobium (<0.01% vs. 0.06%). Our findings indicate that rumen microbiota transfaunation shifts rumen fermentation toward acetate and butyrate production through a change in the rumen microbial composition in Japanese Black cattle.

摘要译文

【摘要】该研究旨在研究瘤胃微生物群体转移对日本黑牛的生长，瘤胃发酵和微生物群落的影响，这些牛早期与大坝分离。在这里，产犊后立即将24只小牛与大坝分离，其中12个是通过接种成年牛的瘤胃液通过2个月大的瘤胃液转移的，其余12个则保持不合同（未接种）。在7-10个月大时进行饲料效率监测。体重和进食摄入量之间的体重和饲料摄入量没有显着差异。转染增加了乙酸盐和丁酸酯的相对水平，但降低了丙酸酯的水平，从而增加了非葡萄糖/糖原性短链脂肪酸比率。微生物16s，18s及其核糖体RNA基因扩增子分析表明，转移和不繁殖的牛之间的瘤胃微生物多样性和成分有所不同。转染增加了乙酸酯和丁酸酯产生的细菌的丰度，并降低了与丙酸丙酸酯产生相关的细菌属的丰度。转料也增加了甲烷二氨基菌的丰度（1.94％vs. 0.05％）和Neocallimastix（27.1％vs. 6.8％），但甲烷菌粒菌的丰度（<0.01％vs. 0.06％）降低。我们的发现表明，瘤胃微生物群的转移会通过改变日本黑牛的瘤胃微生物组成的变化，将瘤胃发酵向乙酸和丁酸酯的产生。

Shuhei Takizawa (0000-0001-5588-0736) [1];Takumi Shinkai (0000-0002-1849-557X) [2];Kunihiko Saito [3];Natsuko Fukumoto [4];Yukari Arai [5];Tomokazu Hirai [6];Masaharu Maruyama [7];Masayuki Takeda (0000-0002-0363-9411) [8];. Effect of rumen microbiota transfaunation on the growth, rumen fermentation, and microbial community of early separated Japanese Black cattle[J]. Animal Science Journal, 2023,94(1)