摘要
We report the complete genome sequence of a novel mucolytic bacterium, Prevotella mucinisolvens sp. nov. Mucolytic bacteria were isolated from rumen epithelium of the dorsal sac of Korean cattle steer using a targeted cultivation on a mucin defined medium as a sole carbon source in anaerobic conditions. The genome of P. mucinisolvens was sequenced by means of both the Illumina HiSeq™ X and PacBio RSII platforms. The genome (2,730,135 bp) was found to contain 2,445 genes, 2,374 coding sequences, 61 transfer RNA, 1 transfer-messenger RNA, and 9 ribosomal RNA. The P. mucinisolvens had a total 51 glycoside hydrolases (GHs), of which 14 GHs, including β-galactosidases (GH2, GH20), α-N-acetylgalactosaminidases (GH101), α-N-acetylglucosaminidase (GH89), sialidase (GH33), and fucosidases (GH29, GH95), were identified as enzymes involved in mucin degradation. Following the KEGG pathways, the putative mucolytic pathway was constructed, including the metabolism of carbon sources such as galactose, N-acetylglucosamine, sialic acid (N-acetylneuraminic acid), and mannose. The presence of putative extracellular polysaccharide biosynthesis pathways, including Wzx/Wzy-dependent pathway (4 putative glycosyltransferases, 3 acetyltransferases, 1 flippase, 1 polymerase, 1 polysaccharide co-polymerase, and 1 outer membrane transport protein) and synthase-dependent pathway (1 putative synthase, 3 precursors of synthesis), was confirmed in P. mucinisolvens. Twelve putative virulence factors associated with adherence (hasB, KpsF, and htpB), stress reactions (clpP and clpC), antiphagocytosis (hasB and bcs1), O-antigen (gmd, fcl, and galE), and metabolic adaption (panD) were identified. This study contributes to a better understanding of epimural bacteria in putative mucin-degrading ability.
摘要译文
我们报告了一种新型的粘液溶液prevotella粘菌素sp。的完整基因组序列。十一月。在厌氧条件下，在粘蛋白定义的培养基上，使用靶向培养在肌肉蛋白上定义为唯一的碳源，从韩国牛转导的瘤胃上皮中分离出粘液溶细菌。 P.粘菌素的基因组通过Illumina Hiseq™X和PACBIO RSII平台进行了测序。发现基因组（2,730,135 bp）包含2,445个基因，2,374个编码序列，61个转移RNA，1个转移 - 符号RNA和9个核糖体RNA。 The P. mucinisolvens had a total 51 glycoside hydrolases (GHs), of which 14 GHs, including β-galactosidases (GH2, GH20), α-N-acetylgalactosaminidases (GH101), α-N-acetylglucosaminidase (GH89), sialidase (GH33 ）和岩藻糖苷酶（GH29，GH95）被鉴定为参与粘蛋白降解的酶。遵循KEGG途径，构建了推定的粘液溶解途径，包括碳源的代谢，例如半乳糖，N-乙酰葡萄糖，唾液酸（N-乙酰基神经氨酸）和MANNOSE。假定的细胞外多糖生物合成途径的存在，包括WZX/WZY依赖性途径（4个假定的糖基转移酶，3个乙酰基转移酶，1个FLIPPase，1个聚合酶，1个多糖含量糖浆二糖偶联酶酶，和1个外膜蛋白质和1个依赖性蛋白（1）假定的合成酶是合成的3个前体），在粘粘剂疟原虫中得到了证实。与依从性（Hasb，KPSF和HTPB）相关的十二个推定的毒力因子，压力反应（CLPP和CLPC），抗吞噬作用（Hasb和BCS1），O-抗原（GMD，FCL和GALE）以及代谢适应（PAND）是确定。这项研究有助于更好地了解假定的粘蛋白降解能力中的表皮细菌。