摘要
Previous work has shown that in a cell-free system from Escherichia coli, bacteriophage f2 RNA initiates the synthesis of three proteins and that two of these are the phage coat protein and RNA polymerase. We prove now that the third protein, comprising 1 to 2% of the total protein produced, is the maturation protein, the product of the third phage gene. Fingerprinting and acrylamide gel techniques, as well as mutant and wild-type RNA messengers, were used. We confirm our earlier notion that synthesis of maturation protein is independent of translation both of coat and polymerase genes, a result which implies that there must be at least two sites on f2 RNA to which ribosomes can attach independently.
摘要译文
以前的工作表明，在来自大肠杆菌的无细胞系统中，噬菌体f2 RNA启动三种蛋白质的合成，其中两种是噬菌体外壳蛋白和RNA聚合酶。我们现在证明，第三种蛋白质，包括产生的总蛋白质的1-2％，是成熟蛋白质，第三种噬菌体基因的产物。使用指纹图谱和丙烯酰胺凝胶技术，以及突变体和野生型RNA信使。我们证实了我们早先的观点，即成熟蛋白的合成不依赖于外壳和聚合酶基因的翻译，这一结果暗示在核糖体可以独立附着的f2 RNA上必须存在至少两个位点。
Harvey F.Lodish[a][b];Hugh D.Robertson[a][b];. Cell-free synthesis of bacteriophage f2 maturation protein[J]. Journal of Molecular Biology, 1969,45(1): 9-12, IN1, 13-22