摘要
【Abstract】 Cell-free expression is a technique used to synthesize proteins without utilising living cells. This technique relies mainly on the cellular machinery —ribosomes, enzymes, and other components — extracted from cells to produce proteins in vitro. Thus far, cell-free expression systems have been used for an array of biologically important purposes, such as studying protein functions and interactions, designing synthetic pathways, and producing novel proteins and enzymes. In this review article, we aim to provide bacteriophage (phage) researchers with an understanding of the cell-free expression process and the potential it holds to accelerate phage production and engineering for phage therapy and other applications. Throughout the review, we summarize the system’s main steps and components, both generally and particularly for the self-assembly and engineering of phages and discuss their potential optimization for better protein and phage production. Cell-free expression systems have the potential to serve as a platform for the biosynthetic production of personalized phage therapeutics. This is an area of in vitro biosynthesis that is becoming increasingly attractive, given the current high interest in phages and their promising potential role in the fight against antimicrobial resistant infections.
摘要译文
【摘要】无细胞表达是一种用于合成蛋白质而无需使用活细胞的技术。该技术主要依赖于细胞机制（ribosomes，酶和其他成分）从细胞中提取以在体外产生蛋白质。迄今为止，无细胞表达系统已用于一系列重要的生物学重要目的，例如研究蛋白质功能和相互作用，设计合成途径以及产生新型蛋白质和酶。在这篇评论文章中，我们旨在为噬菌体（噬菌体）研究人员了解无细胞的表达过程及其在加速噬菌体疗法和其他应用的潜力。在整个综述中，我们总结了系统的主要步骤和组件，尤其是对于噬菌体的自组装和工程，并讨论了它们对更好的蛋白质和噬菌体生产的潜在优化。无细胞表达系统有可能作为个性化噬菌体疗法生物合成生产的平台。鉴于当前对噬菌体的浓厚兴趣及其在抵抗抗菌抗药性感染中的斗争中，这是一个体外生物合成的领域，它变得越来越有吸引力。
Hanzada Nour El-Din [1];Maryam Kettal [2];Serena Lam [3];José Granados Maciel [4];Danielle L. Peters [5];Wangxue Chen [6];. Cell-free expression system: a promising platform for bacteriophage production and engineering[J]. Microbial Cell Factories, 2025,24(1): 42