摘要
To date, the number of green sea and hawksbill sea turtles is in decline due to environmental, anthropogenic, and pathological factors. The present study described the pharmacokinetic characteristics of danofloxacin (DNX) in green sea and hawksbill sea turtles, following single intravenous (i.v.) and intramuscular (i.m.) administrations at single dosages of 6 mg/kg body weight (b.w.). Blood samples were collected at assigned times up to 168 h. DNX in the harvested plasma was cleaned up using liquid–liquid extraction and analyzed using a validated high-performance liquid chromatography method with fluorescence detection. The pharmacokinetic analysis was performed using a non-compartmental approach. DNX was quantifiable from 5 min to 168 h after i.v. and i.m. administrations at an identical dosage in both turtle types. No statistical differences were found in the pharmacokinetic parameters between green sea and hawksbill sea turtles. The long elimination half-life value of DNX obtained in green sea (35 h) and hawksbill sea (30.21 h) turtles was consistent with the quite large volume of distribution and the slow clearance rate. The high values of absolute bioavailability (87%–94%) should be advantageous for clinical use of DNX in sea turtles. According to the pharmacokinetic–pharmacodynamic surrogate (AUC0-24/MIC > 125), DNX is predicted to have antibacterial success for disease caused by bacteria with MIC < 0.04 μg/ml.
摘要译文
迄今为止，由于环境，人为因素和病理因素，绿海和鹰海龟的数量正在下降。本研究描述了绿海和鹰海龟中丹诺福沙星（DNX）的药代动力学特征，在单次静脉内（i.v.）和肌内（I.M.）施用后，单剂量为6 mg/kg体重（B.W.）。在分配的时间内收集血液样本最多168小时。使用液态液萃取清理收获的血浆中的DNX，并使用经过验证的高性能液相色谱法分析了荧光检测。使用非室内方法进行药代动力学分析。静脉内5分钟至168小时可量化DNX。和I.M.两种乌龟类型的剂量相同剂量的管理。在绿海和鹰海龟之间的药代动力学参数中未发现统计差异。在绿海（35小时）和鹰海（30.21 h）海龟获得的DNX的长期消除半衰期值与相当大的分布和缓慢的间隙速率一致。绝对生物利用度的高值（87％–94％）对于在海龟中临床使用DNX应该是有利的。根据药代动力学 - 药力动力替代（AUC 0-24 /MIC> 125），预计DNX被预测是由MIC <0.04μg /mL的细菌引起的疾病抗菌成功。
Wipasitnee Wanmad[1];Thanaphan Chomcheun[2];Oranee Jongkolpath[2];Narumol Klangkaew[1];Napasorn Phaochoosak[1];Rattapong Sukkheewan[1];Weerapong Laovechprasit[2];Kraisiri Khidkhan[1];Mario Giorgi[3];Amnart Poapolathep[1];Saranya Poapolathep[1]. Pharmacokinetic characteristics of danofloxacin in green sea (Chelonia mydas) and hawksbill sea (Eretmochelys imbricata) turtles[J]. Journal of Veterinary Pharmacology and Therapeutics, 2022,45(4): 402-408