摘要
The objectives of this study were to investigate the shifts in rumen and colon mucosa-associated microbiota in dairy calves fed a high milk replacer feeding rate before and after weaning and to determine whether such shifts are associated with tissue physiological measures. Longitudinal biopsy was performed to collect rumen and colon mucosal tissues of 4 ruminally cannulated Holstein dairy bull calves (weaned at 6 wk of age) at the end of wk 5 (before weaning), 7 (weaning adaptation) and 12 (after weaning), and were used to assess mucosa-associated microbiota and their changes using amplicon sequencing. Both rumen and colon mucosa-associated bacterial communities shifted during the weaning process, as evidenced by their clear separation among 3 different weaning periods and increased α diversity (Shannon and Chao1 indices) during weaning transition. Among the 3 dominant bacterial phyla identified (relative abundance >1.0%), the relative abundance of Proteobacteria and Bacteroidetes decreased in the rumen mucosa, whereas the relative abundance of Firmicutes increased in both rumen and colon mucosa during weaning transition. In the rumen mucosa, Campylobacter (0.6–22.1%) gradually became prevalent during weaning transition, whereas Succinivibrio (6.2–10.3%) and Prevotella 1 (4.7–10.5%) were dominant regardless of weaning transition. In the colon mucosa, Bacteroides (12.8–25.4%) was dominant during weaning transition, although its relative abundance decreased after weaning. In the meantime, relative abundance of uncultured Lachnospiraceae increased from 2.2% to 25.7% during this period. In addition, genera Pyramidobacter (in the rumen mucosa) and Lachnoclostridium (in the colon mucosa) were positively correlated with rumen papilla surface area and colon mucosal thickness, respectively. Moreover, genera Ruminococcaceae UCG-005 and Sharpea in the rumen mucosa were positively correlated with the molar proportion of propionate and butyrate, respectively. Overall, our findings revealed that rumen and colon mucosa-associated bacterial communities altered in response to the weaning transition, and some bacterial taxa in these communities may have positive effects on rumen and colon mucosa development during this period.
摘要译文
本研究的目的是探讨瘤胃牛犊中瘤胃和结肠粘膜相关微生物群的乳牛犊，在断奶前后喂养高牛奶替代品喂养率，并确定这种换档是否与组织生理措施相关。在WK 5（断奶前），7（断奶后）和12（断奶后）和12（断奶后）和12（断奶后）和结肠粘膜组织中进行瘤胃和结肠粘膜组织并且用于评估粘膜相关的微生物群及其使用扩增子测序的变化。瘤胃和结肠粘膜相关的细菌社区在断奶过程中移位，如在断奶转换期间的3种不同断奶期和增加的α多样性（Shannon和Chao1索引）之间的清晰分离所证明。在鉴定的3种显性细菌植物中（相对丰度> 1.0％）中，瘤胃粘膜中的噬菌体和菌斑的相对丰度降低，而断奶转变期间瘤胃和结肠粘膜的相对丰度增加。在瘤胃粘膜中，弯曲杆菌（0.6-22.1％）在断奶过渡期间逐渐变得普遍，而琥珀酰纤维（6.2-10.3％）和PREVOTLA 1（4.7-10.5％）是显着的，无论发生断奶过渡。在结肠粘膜中，在断奶过渡期间，拟枝杆菌（12.8-25.4％）占优势，尽管断奶后其相对丰度降低。与此同时，在此期间期间，未培养的Lachnospheae的相对丰度从2.2％增加到25.7％。此外，Genera Pyramobacter（在瘤胃粘膜中）和Lachnoclostridium（在结肠粘膜中）分别与瘤胃乳头表面积和结肠粘膜厚度呈正相关。此外，瘤胃粘膜中的Genera喇菇和Shaprea分别与丙酸酯和丁酸丁酸的摩尔比例呈正相关。总体而言，我们的研究结果揭示了瘤胃和结肠粘膜相关的细菌社区因响应断奶转型而改变，这些社区的一些细菌分类群可能对瘤胃和结肠粘膜发育产生积极影响。
W.Guo[1][2];J.K.van Niekerk[2];M.Zhou[2];M.A.Steele[3]. Longitudinal assessment revealed the shifts in rumen and colon mucosal-attached microbiota of dairy calves during weaning transition[J]. Journal of Dairy Science, 2021,104(5)