摘要
In this study, the plasma non-targeted metabolomics of Yili horses were characterized before and after exercise on tracks that differed in surface hardness to better understand exercise-related biochemical changes. Blood samples were obtained from eight trained Yili horses before and immediately after exercise. Samples were used for metabolomic analysis by ultra-performance liquid chromatography-Q-EXACTIVE mass spectrometry. In total, 938 significantly different metabolites involving sugar, lipid, and amino acid metabolism were detected in the plasma, with significant increases in glucose, glucoheptanoic acid, lactic acid, malic acid, and methylmalonic acid and significant decreases in creatinine, D-tryptophan, carnitine, and citric acid after exercise. Among these metabolites, acetylcarnitine, tuliposide, vitamin C, and methylmalonic acid showed regular changes in concentration after exercise on tracks that differed in surface hardness, providing new insights into equine exercise physiology. The findings indicated the potential of vitamin C and methylmalonic acid as novel biomarkers of equine locomotor injury.
摘要译文
在这项研究中，在锻炼前后，在表面硬度不同，以更好地理解与运动相关的生物化学变化的轨道上的YILI马的血浆非靶向代谢组学的表征。运动前后从八匹训练的YILI马获得血液样本。样品通过超表现液相色谱 - Q激活质谱法进行代谢组学分析。总共938个显着不同的代谢产物，涉及糖，脂质和氨基酸代谢，在血浆中检测到葡萄糖，葡萄糖，葡萄糖醇酸，乳酸，苹果酸，苹果酸和甲基甲酸的葡萄糖，葡萄糖酸，甲基酸酸和明显降低的葡萄糖，肌酐，蛋白酶，d- tryptryptophan，dtryptophan，dtryptophan，distopophan，葡萄糖酸，乳酸，乳酸，乳酸，乳糖酸，显着增加。运动后肉碱和柠檬酸。在这些代谢产物中，乙酰炭碱，tuliposide，Vitamin C和甲基甲酸在运动后锻炼后的浓度正常变化，在表面硬度上有所不同，从而为马运动生理提供了新的见解。研究结果表明，维生素C和甲基丙二酸作为马运动损伤的新生物标志物的潜力。
Tongliang Wang; Yaqi Zeng; Chaoxin Ma; Jun Meng; ... Xinkui Yao. Plasma Non-targeted Metabolomics Analysis of Yili Horses Raced on Tracks With Different Surface Hardness[J]. Journal of Equine Veterinary Science, 2023,121