摘要
Mulberry leaves have been used as a protein source in replacing concentrates of domestic animals, however, little is known about the relationship between supplementation level and the development of rumen epithelium. This experiment aimed to investigate the effects of different proportions of mulberry leaf powder (MLP) in dietary concentrate on rumen fermentation and rumen epithelium morphology in fattening Hu sheep. Forty three-month-old male Hu sheep with an initial body weight of 16.5 ± 0.6 kg (BW ± SD) were chosen and randomly divided into five treatments: 0% (control), 15% (T15), 30% (T30), 45% (T45) and 60% (T60) of MLP in concentrate, respectively. The results showed that the dry matter intake (DMI) and average daily gain (ADG) in treatments T15 and T30 have no significant difference with respect to the control treatment, but DMI and ADG in treatments T45 and T60 were lower than the control treatment (p < 0.05). The apparent digestibility of organic matter (OM) and neutral detergent fiber (NDF) increased linearly and quadraticly as MLP supplementation increased (p < 0.05). The concentration of ammonia (NH3-N) trended to decrease linearly with the increase of MLP supplementation (p < 0.1), whereas the microbial protein (MCP) concentration increased linearly as MLP supplementation increased (p < 0.05). In the results of rumen epithelium morphology, the width of stratum corneum was reduced, whereas the width of ruminal papillae increased (p < 0.05), and the width of stratum granulosum and stratum basale also increased as MLP increased. In summary, MLP supplementation could improve nutrient digestibility, the development of rumen papillae and stratum basale. However, high content MLP (45%–60%) supplementation decreased the growth and food intake performance of fattening Hu sheep. Therefore, 30% MLP is recommended to supplement in concentrate for fattening Hu sheep.
摘要译文
桑叶在更换家畜的浓缩物，然而，鲜为人知的是，补充水平和瘤胃上皮发展之间的关系被用作蛋白质来源。本实验旨在探讨桑叶粉（MLP）的不同比例的膳食集中在瘤胃发酵和瘤胃上皮细胞形态育肥湖羊的效果。四十三月龄雄性湖羊的初始体重为16.5±0.6公斤（BW±SD）被选择和随机分成五个处理：0％（对照），15％（T15），30％（T30） ，45％（T45），并在浓缩物分别MLP的60％（T60）。结果表明，在治疗T15和T30的干物质采食量（DMI）和平均日增重（ADG）具有相对于对照处理无显著差异，但DMI和ADG在治疗T45和T60均低于对照治疗下（ p <0.05）。作为补充MLP增加（p <0.05）的有机物质（OM）和中性洗涤纤维（NDF）的表观消化率线性和quadraticly增加。氨的浓度（NH3-N）趋于与MLP补充（P <0.1）的增加而线性减小，而微生物蛋白（MCP）浓度线性增加为MLP补充增加（p <0.05）。在瘤胃上皮形态的结果，角质层的宽度减小，而瘤胃乳头的宽度增加（p <0.05），和颗粒层的宽度和基底层也随着MLP增加。综上所述，MLP补充可以提高营养物质的消化率，瘤胃乳头层和基底层的发展。然而，高含量的MLP（45％-60％）的补充育肥湖羊的生长和食物摄入性能下降。因此，30％的MLP建议补充精矿育肥湖羊。
Jialiang Ouyang[ 1]; Mengzhi Wang[ 1;[*]; Qirui Hou[ 2]; Dan Feng[ 3]; Yu Pi[ 3];Weiguo Zhao[ 2]. Effects of Dietary Mulberry Leaf Powder in Concentrate on the Rumen Fermentation and Ruminal Epithelium in Fattening Hu Sheep[J]. Animals, 2019,9(5): 218