期刊文献

Melatonin protects porcine oocyte from copper exposure potentially by reducing oxidative stress potentially through the Nrf2 pathway 收藏

褪黑激素通过潜在通过NRF2途径减少氧化应激来保护猪卵母细胞免受铜的暴露

原文求助发布源

作者

作者单位

[a]College of Animal Science and Technology, Jilin Agricultural University, Jilin Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Jilin Changchun 130118, China[b]Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, 130118, ChinaKey Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun 130118, China[c]College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin Jilin, 132109, ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin Jilin 132109, China

发布日期

1 November 2022

页码

1-10

DOI

10.1016/j.theriogenology.2022.09.004

来源信息

Theriogenology ISSN：0093-691X, 2022年, 193卷, 1-10页

摘要

Copper is widely used as a feeding additive to promote livestock growth. However, excessive copper can be excreted with feces, causing heavy metal pollution and aggravating environmental problems. At the same time, studies have found that excess copper can cause damage to reproductive function and reduce gamete quality. Here, we explored the effects of adding different concentrations of copper to the culture medium on porcine oocytes. First polar body extrusion rate, embryo development, and intracellular levels of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) ΔΨm, adenosine triphosphate(ATP) content, and acetylation of lysine 9 on histone H3 protein subunit (H3K9ac) were assessed. Results demonstrated that Cu exposure causes abnormalities in mitochondrial function and epigenetic modification, resulting in increased oxidative stress and levels of ROS, ultimately leading to a decreased porcine oocyte quality. In addition, we found melatonin can protect porcine oocytes from those damages. Notably, Nrf2 protein expression was significantly increased by copper exposure, meanwhile, Nrf2 signaling pathway inhibitor ML385 significantly attenuated the protective role of melatonin on oxidative stress induced by copper exposure. In summary, our study demonstrates that copper activates the Nrf2 pathway and impairs oocyte maturation by inducing oxidative stress, leading to poor quality of porcine oocytes, and the changes can be reversed by melatonin.

摘要译文

铜被广泛用作喂养添加剂，以促进牲畜生长。但是，过多的铜可以用粪便排出，从而导致重金属污染并加剧环境问题。同时，研究发现，过量的铜会损害生殖功能并降低配子质量。在这里，我们探索了在猪卵母细胞上向培养基中添加不同浓度的铜的影响。第一极性体挤压率，胚胎发育和细胞内活性氧（ROS），线粒体膜电位（MMP）Δψm，三磷酸腺苷（ATP）含量和酯9对组蛋白H3蛋白亚基（H3K9AC）上赖氨酸9的含量和乙酰化的含量。。结果表明，CU暴露会导致线粒体功能和表观遗传修饰的异常，从而导致氧化应激和ROS水平增加，最终导致猪卵母细胞质量降低。此外，我们发现褪黑激素可以保护猪卵母细胞免受这些损害。值得注意的是，通过铜暴露，NRF2蛋白表达显着增加，与此同时，NRF2信号通路抑制剂ML385显着减弱了褪黑激素对铜暴露诱导的氧化应激的保护作用。总而言之，我们的研究表明，铜激活NRF2途径并通过诱导氧化应激损害卵母细胞成熟，从而导致猪卵母细胞质量差，并且褪黑激素可以逆转。

Chenglin Zhan[a][b];Xu Cao[a][b];Tianrui Zhang[a][b];Jing Guo[a][b];Gaoqing Xu[a][b];Hongyan Wang[c];Wenyan Yang[a][b];Lianyu Yang[a][b];Dongsheng Che[a][b];Wenfa Lu[a][b]PersonEnvelope;Xin Ma[a][b]PersonEnvelope. Melatonin protects porcine oocyte from copper exposure potentially by reducing oxidative stress potentially through the Nrf2 pathway[J]. Theriogenology, 2022,193: 1-10