摘要
To date, the role of ruminal epithelial attached microbiota in cattle feed efficiency is undefined. In this study, we aimed to characterize transcriptionally active bacteria and archaea attached to the rumen epithelial wall and to determine whether they differ in cattle with varied feed efficiency. RNA-sequencing was performed to obtain the rumen epithelial transcriptomes from 9 of the most efficient (low RFI) and 9 of the most inefficient (high RFI) animals. The bacteria and archaea 16S rRNA transcripts were identified using an in-house developed pipeline, enriched from filtered reads that did not map to the bovine genome. Archaea from unclassified genera belonging to the Euryarchaeota phylum showed the most activity on the rumen epithelium of low RFI (81.3 ± 1.9%) and high RFI (76.4 ± 3.0%) steers. Bacteria from the Succinivibrionaceae family showed the greatest activity of bacteria on the low RFI (28.7 ± 9.0%) and high RFI (33.9± 8.8%) epithelium. Of the bacterial families, Campylobacteraceae and Neisseriaceae had significantly greater activity on the low RFI epithelium (p < 0.05) and are known to play a role in oxygen scavenging. Greater activity of rumen epithelial attached oxygen scavenging bacteria may provide more optimal feed fermentation conditions, which contributes to high fermentation efficiency in the rumen.
摘要译文
迄今为止，瘤胃上皮外皮DIROBIOTA在牛饲料效率中的作用是未定义的。在这项研究中，我们的目标是在瘤胃上皮壁附着的转录活性细菌和古痤疮，并确定它们是否在牛中不同的饲料效率。进行RNA测序以获得从最有效（低RFI）和最低效率（高RFI）动物的9个中的瘤胃上皮转录om。使用内部开发的管道鉴定细菌和古痤疮16S rRNA转录物，从未映射到牛基因组的过滤的读数中富集。来自euryArchaeota Phylum的未分类属的古亚ea在低RFI（81.3±1.9％）和高RFI（76.4±3.0％）操纵器中，瘤胃上皮的最多活性。来自琥珀素纤维的细菌家族展示了低RFI（28.7±9.0％）和高RFI（33.9±8.8％）上皮的最大活性。在细菌家族中，弯曲杆菌和NeisseriaCeae在低RFI上皮上具有显着更大的活性（P <0.05），已知在氧气清除中发挥作用。瘤胃上皮附加的氧清除细菌的更大活性可以提供更优异的饲料发酵条件，这有助于瘤胃中的发酵效率。
Rebecca S.G. Tan[a][1];Mi Zhou[a][1];Fuyong Li[a];Le Luo Guan[a]. Identifying active rumen epithelial associated bacteria and archaea in beef cattle divergent in feed efficiency using total RNA-seq[J]. Current Research in Microbial Sciences, 2021,2