摘要
A decade since its invention, single-cell RNA sequencing (scRNA-seq) has become a mainstay technology for profiling transcriptional heterogeneity in individual cells. Yet, most existing scRNA-seq methods capture only polyadenylated mRNA to avoid the cost of sequencing non-messenger transcripts, such as ribosomal RNA (rRNA), that are usually not of-interest. Hence, there are not very many protocols that enable single-cell analysis of total RNA. We adapted a method called DASH (Depletion of Abunda
摘要译文
自发明以来，单细胞RNA测序（SCRNA-SEQ）已成为用于分析单个细胞中的转录异质性的主干技术。然而，大多数现有的SCRNA-SEQ方法仅捕获多腺苷酸化的mRNA，以避免通常不感兴趣的核糖体RNA（RRNA）等非信使转录物的成本。因此，没有很多协议，可以实现总RNA的单细胞分析。我们改编了一种称为划分的方法（耗尽Abunda
Danson S.C. Loi; Lei Yu; ; Angela R. Wu. Effective ribosomal RNA depletion for single-cell total RNA-seq by scDASH[J]. PeerJ, 2021,9