摘要
The objective of this research is to investigate the effect of yeast (Saccharomyces cerevisiae) adding and roughage-to-concentrate ratio (R:C ratio) on nutrients utilization, rumen fermentation efficiency, microbial protein synthesis, and protozoal population in Thai native beef cattle. Four Thai native beef cattle, weighing an average of 120 ± 10 kg live weight, were randomly assigned to four dietary treatments using a 2 × 2 factorial arrangement in a 4 × 4 Latin square design. Factor A was the level of roughage-to-concentrate ratio (R:C ratio) at 60:40 and 40:60; factor B was the levels of live yeast (LY) supplementation at 0 and 4 g/hd/d; urea–calcium-hydroxide-treated rice straw were used as a roughage source. Findings revealed that total intake and digestibility of dry matter (DM), organic matter (OM), and crude protein (CP) were increased (p < 0.05) by both factors, being greater for steers fed a R:C ratio of 40:60 supplemented with 4 g LY/hd/d. Ruminal ammonia nitrogen, total volatile fatty acid (VFA), and propionate (C3) were increased (p < 0.05) at the R:C ratio of 40:60 with LY supplementation at 4 g/hd/d, whereas rumen acetate (C2) and the C2 to C3 ratio were decreased (p < 0.05). With a high level of concentrate, LY addition increased total bacterial direct counts and fungal zoospores (p < 0.05), but decreased protozoal populations (p < 0.05). High-concentrate diet and LY supplementation increased nitrogen absorption and the efficiency of microbial nitrogen protein production. In conclusion, feeding beef cattle with 4 g/hd/d LY at a R:C ratio of 40:60 increased C3 and nutritional digestibility while lowering protozoal population.
摘要译文
本研究的目的是探讨酵母(酿酒酵母)添加和粗致浓缩比(R:C比率)对泰国天然肉牛的营养利用,瘤胃发酵效率,微生物蛋白质合成和原生动物种群的影响。四个泰国本土牛粪,平均重量为120±10公斤的活力,随机分配到四个膳食处理,使用4×4拉丁方形设计中的2×2因子排列。因子A是60:40和40:60的粗致浓缩比(R:C比率)的水平;因子B是0和4g / hd / d的活酵母(ly)补充的水平;尿素 - 氢氧化钙处理的稻草用作粗源。结果表明,两种因素增加了干物质(DM),有机物(OM),有机物(OM)和粗蛋白(CP)的总摄入和消化率(P <0.05),喂养R:C比例为40的R:C比率更大: 60辅以4 G LY / HD / D。瘤胃氨氮,总挥发性脂肪酸(VFA)和丙酸盐(C3)在R:C比率为40:60,含量为4g / hd / d,而甲酸瘤(C2 )和C2至C3比率降低(P <0.05)。具有高浓度的浓缩物,加入总细菌直接计数和真菌动物孢子(P <0.05),但原生动物种群减少(P <0.05)。高浓缩饮食和补充补充提高了氮吸收和微生物氮蛋白质产生的效率。总之,用4g / hd / d ly喂养牛肉牛,r:c比率为40:60增加C3和营养消化率,同时降低原生动物群。
Kampanat Phesatcha;Burarat Phesatcha;Metha Wanapat;Anusorn Cherdthong. The Effect of Yeast and Roughage Concentrate Ratio on Ruminal pH and Protozoal Population in Thai Native Beef Cattle[J]. Animals, 2022,12(1): 53