摘要
This study investigated whether specific proteins from distinct seminal plasma fractions of boars could be related to in vivo fertility. Nine boars with acceptable sperm motility and morphology for use in artificial insemination demonstrated major differences in total number born and pregnancy rate when low sperm doses (1.5 billion sperm) were used to breed a minimum of 50 gilts per boar. The 2 lowest-fertility and 2 highest-fertility boars were chosen for evaluation of specific seminal plasma proteins. On 4 occasions, semen was collected and separated into 3 fractions based on sperm concentration (Sperm-Peak, Sperm-Rich, and Sperm-Free), and the fractions were analyzed for total protein concentration and abundance of major seminal plasma glycoprotein (PSP-I), AWN-1, and osteopontin protein using Western blotting techniques. The concentrations of these seminal plasma proteins were lower in the Sperm-Peak fractions compared with the Sperm-Free fractions (P < .05). Seminal plasma from the pooled Sperm-Rich fraction used for artificial insemination was also subjected to two-dimensional gel electrophoresis to investigate novel protein markers related to in vivo fertility. Total piglets born (r = −0.76, P = .01) and sperm motility at day 7 (r = −0.74, P = .037) were again negatively correlated with a 22-kDa protein identified by mass spectrometry as PSP-I. However, fertility index and farrowing rate tended to be positively correlated (P < .10) with a 25-kDa protein, identified as glutathione peroxidase (GPX5), an antioxidant enzyme that may protect sperm membranes from oxidative damage. These candidate proteins merit further investigation as markers of fertility in boars.
摘要译文
本研究调查了来自公猪不同精液血浆部分的特异性蛋白质是否可能与体内生育力有关在精子数量低的情况下，有9只具有可接受的精子活力和形态学用于人工授精的公猪表现出总出生率和妊娠率的主要差异（1 五十万个精子）用于每公顷育种至少50只母猪。选择2种最低生殖力和2种最高生育力来评估特异性精浆血浆蛋白质4次，收集精液并根据精子浓度（精子峰，精子和无精子）分为3个部分，并使用Western印迹技术分析各组分的总蛋白浓度和主要精浆血糖（PSP-I），AWN-1和骨桥蛋白的丰度与无精子级分相比，这些精液血浆蛋白的浓度在精子峰值部分较低（P 来自用于人工授精的合并的精子富集级别的血浆也进行二维凝胶电泳以研究与体内生育力相关的新型蛋白质标记总共出生的仔猪（r \x3d鈭6，P \x3d 和第7天的精子活力（r \x3d鈭4，P \x3d ）与通过质谱鉴定的22-kDa蛋白质再次呈负相关，如PSP-I ，生育指数和分娩率呈正相关（P 与25kDa蛋白，鉴定为谷胱甘肽过氧化物酶（GPX5），一种可以保护精子膜免受氧化损伤的抗氧化酶。这些候选蛋白质值得进一步研究，作为公猪生殖力的标志物
Susan Novak[†]; Ana Ruiz-Sánchez[† Walter T. Dixon, George R. Foxcroft and Dr Michael K. Dyck[*];. Seminal Plasma Proteins as Potential Markers of Relative Fertility in Boars[J]. Journal of Andrology, 2010,31(2): 188-200