摘要
Using a couple of Husky Siberian, it is described for the first time in Chile a kind of artificial insemination using cooled canine semen. The semen was obtained by digital manipulation and was diluted with UHT semi-skimmed milk and antibiotics in relation 1:4 and cooled at 5oC. There inseminations were carried out on the third day of the oestrus which was determined through vaginal cytology, considering the beginning of the oestrus when the superficial cells constituted over the 80% of the total vaginal cells in the vaginal smear. Inseminated was carried out using cooled doses for 24 and 48 hours and with an average concentration of 600 x 106 spermatozoa. The pregnancy diagnosis, through a real time ultrasonography, was done 28 days after the last insemination and the bitch gave birth to 4 normal puppies 61 days after last insemination
摘要译文
使用几种赫斯基西伯利亚人，首次在智利描述了一种使用冷冻犬精液的人工授精。精液通过数字操作获得，并用UHT半脱脂乳和抗生素稀释1：4和在5℃冷却。在通过阴道细胞学测定的发情的第三天进行了授精，考虑到当阴道涂片中总表面细胞占总阴道细胞的80％25的表面细胞时，发情的开始被发现使用冷却剂量进行24和48小时，平均浓度为600×10 6精子。通过实时超声检查，在最后一次授精后28天完成了妊娠诊断，在最后一次授精后61天，bit子生4只正常小狗
SANCHEZ, A. e RUBILAR, J... Puppies obtained using artificial insemination with chilled extended semen.: First report in Chile.[J]. Archivos de medicina veterinaria, 2001,33(1): 105-110