摘要
The replication of the double-stranded replicative-form DNA of bacteriophage M-13 was studied in a cellular system in vitro prepared by plasmolysis of M-13-am5-infected Escherichia coli cells. Newly synthesized DNA was density-labelled with bromodeoxyuridine triphosphate and analysed by equilibrium centrifugation in neutral CsCI. After a 60-min incubation at 30°C 15 – 20% of the radioactive label incorporated from [32P]dGTP was found in fully synthetic (duplex DNA, corresponding to 7–9 replicative form molecules cell. The plasmolysed cell system is therefore capable of re-initiating new rounds of replicative form replication in vitro. The kinetics of labelling indicate that molecules are selected for replication at random from an intracellular pool of approximately 150 replicative form molecules. Rifampicin and nalidixic acid, which interfere with the semiconservative replication of replicative form DNA, completely prevent the formation of fully synthetic duplex DNA. .overlined { text-decoration: overline; } .struck { text-decoration:line-through; } .underlined { text-decoration:underline; } .doubleUnderlined { text-decoration:underline;border-bottom:1px solid #000; } View Full Article (HTML) Enhanced Article (HTML) Get PDF (599K)Get PDF (599K) More content like thisFind more content: like this articleFind more content written by: Barbara E. KESSLER-LIEBSCHER Walter L. STAUDENBAUER All Authors
摘要译文
噬菌体的M-13的双链复制型DNA的复制在由M-13-AM5感染大肠杆菌细胞的质壁分离制备体外的蜂窝系统进行了研究新合成的DNA密度标记用溴脱氧尿苷三磷酸和通过在中性氯化铯平衡离心进行分析在30℃下15 60分钟温育后 - 从结合的放射性标记的20％25 SUP 32 / SUP P] dGTP在全合成（双链DNA发现对应7鈥eplicative形式的分子cellThe plasmolysed电池系统因此能够重新启动在体外复制形式复制的新一轮标记的动力学表明，分子从约150复制形式moleculesRifampicin的细胞内池和萘啶酸选择用于复制随机地该干扰复制型DNA的半保留复制，完全阻止全合成的双链DNA的形成竜verlined {文字修饰：上划线; }击中{文字修饰：线通过; }竨nderlined {文本装饰：下划线; }竏oubleUnderlined {文本装饰：下划线;下边框：1px的固体＃000;}查看全文（HTML）增强文章（HTML）获取PDF（599K）获取PDF（599K）更多内容，如thisFind更多的内容：像这样articleFind更多的内容写成：芭芭拉·êKESSLER-利布舍尔沃尔特LSTAUDENBAUER所有的著者
Barbara E. KESSLER-LIEBSCHER and Walter L. STAUDENBAUER. Replication of M-13 DNA in Plasmolysed Escherichia coli Cells Formation of Fully Synthetic Duplex DNA[J]. FEBS Journal, 1976,70(2): 523-529