摘要
The research was undertaken to develop a successful nonsurgical procedure for artificially inseminating ferrets. A fiberoptic endoscope used in conjunction with a specially designed speculum and catheter permitted cervical catheterization and intrauterine insemination. Sperm were collected from the cauda epididymides of 10 discarded breeder males; the number of sperm in diluted samples used for insemination ranged from 4.4–13.6 × 106/100 μl with progressive motility of sperm ranging from 40 to 60%. Sperm collected from each male were diluted with an egg-yolk extender (TEST) and used to inseminate 8–12 females, with deposition of sperm intravaginally or transcervically into the uterine body 0 or 24 hr after an ovulatory injection of human chorionic gonadotropin (hCG). The vaginal inseminations were used as a control, and no pregnancies resulted after insemination of 26 females. Intrauterine inseminations resulted in 4/24 (17%) of the ferrets pregnant when hCG administration was coincident with insemination, and 19/24 (79%) of the ferrets were pregnant when inseminations were done 24 hr after hCG administration. All inseminated females were euthanized on day 20 after insemination to count fetuses. The mean number of fetuses was 3.1 (range, 1–8). The number (millions) of motile sperm inseminated (X) had a significant effect on the percentage of fetuses (Y). Regression analysis indicated a linear relationship between the two variables, with an R2 value of 0.99 and a line of best fit described by the equation Y = 0.029 + 0.034 X. This paper is the first report of transcervical artificial insemination in the domestic ferret (Mustela putorius furo). The method can serve as a model for application to ferrets and other mammals, particularly endangered species. Zoo Biol 17:393–404, 1998. © 1998 Wiley-Liss, Inc. .overlined { text-decoration: overline; } .struck { text-decoration:line-through; } .underlined { text-decoration:underline; } .doubleUnderlined { text-decoration:underline;border-bottom:1px solid #000; } 3.0.CO;2-9/epdf">Get PDF (136K)3.0.CO;2-9/pdf">Get PDF (136K) More content like thisFind more content: 3.0.CO;2-9&scope=allContent&start=1&resultsPerPage=20">like this articleFind more content written by: J. D. Kidder R. H. Foote M. E. Richmond All Authors
摘要译文
研究的目的是为人工授精雪貂研制一种成功的非手术程序。与特别设计的窥器和导管结合使用的光纤内窥镜允许宫颈插管和子宫内授精。从10只废弃的育种雄性的尾部附睾中收集精子;用于授精的稀释样品中的精子数量范围为4.4-13。6×10 6 /100μl，精子进行性运动，从40％到60％不等。从每只雄性采集的精子用蛋黄增量剂（TEST）稀释并用于8-12只雌性受精，在排卵注射人绒毛膜促性腺激素（hCG）后0或24小时，阴道内或跨膜地将精子沉积到子宫体中。用阴道分娩作为对照，26名女性受精后没有怀孕。当hCG给药与受精同时，子宫内授精导致4/24（17％）的雪貂怀孕，和19/24（79％）的雪貂在hCG给药后24小时进行授精时怀孕。在授精后第20天将所有受精的雌性动物安乐死以计数胎儿。平均胎儿数为3.1（范围1-8）。能动精子数量（百万）（X）对胎儿（Y）的百分比有显着影响。回归分析表明这两个变量之间的线性关系，其中R2 \x3d 0.99的值和通过方程Y \x3d 0.029 + 0.034X描述的最佳拟合线。本文是国内首次报道宫颈人工授精（Mustela putorius furo）。该方法可以作为应用于雪貂和其他哺乳动物的模型，[特别是濒危物种。 Zoo Biol 17：393-404,1998。©1998 Wiley-Liss，Inc. .overlined {text-decoration：overline; } .struck {text-decoration：line-through; }。][下划线（文字修饰：下划线; } .doubleUnderlined {text-decoration：underline; border-bottom：1px solid＃000; } 3.0.CO; 2-9 / epdf“获取PDF（136K）3.0.CO;][2-9 / pdf“获取PDF（136K）更多类似这样的内容查找更多内容：3.0.CO; 2-9＆scope \x3d allContent＆start \x3d 1＆resultsPerPage \x3d 20” like this article查找更多内容撰写者：J. D. Kidder R. H. Foote M. E.]Richmond所有的着者/
J. D. Kidder[1]; R. H. Foote[1,*] and M. E. Richmond[2];. Transcervical artificial insemination in the domestic ferret (Mustela putorius furo)[J]. Zoo Biology, 1998,17(5): 393-404