摘要
Since the conclusion of data collation from previously published work, a further 352 inseminations using frozen–thawed dog semen by endoscopic-assisted transcervical insemination (EIU) have been performed by the author. Insemination was performed on the second day in which crenulation of the anterior vagina was detected in conjunction with a progesterone concentration of >10 ng/ml. All semen samples were analysed for total number of sperm, total motility and progressive motility using computer-assisted semen analysis (CASA). The insemination dose was based on the progressively motile normal spermatozoa (PMNS). Insemination was performed on all bitches as previously described using a ureterorenoscope. Additional extender was inseminated subsequent to the semen to expand and fill the uterus. The semen and additional extender were inseminated slowly over a period of 15–20 min. Pregnancy was determined by B-mode ultrasound equipped with a 7.5-MHz probe whilst standing and/or via the whelping rate. The number of sperm inseminated ranged from 9 × 106 PMNS to 519 × 106 PMNS, with progressive motility values ranging between 20% and 80%. The overall pregnancy rate was 68% (238/352). When stratified by PMNS, pregnancy rates were as follows: >150 × 106 PMNS - 76% (110/145), 100–150 × 106 - 68% (87/128) and <100 × 106 PMNS - 52% (41/79). Pregnancy rate was significantly higher when >150 × 106 PMNS (p = .003) or 100–150 ×106 PMNS (p = .027) were inseminated compared to <100 × 106 PMNS. These data are concordant with previous reports recommending the insemination of >150 × 106 PMNS to maximize pregnancy rate. These results indicate that one optimally timed EIU insemination results in similar pregnancy rates to previous publications of one optimally timed, or two or more non-optimally timed inseminations using the Norwegian catheter.
摘要译文
自从以前发表的工作结束数据整理以来，作者通过内镜辅助子宫颈授精（EIU）进一步进行352次使用冻结的狗精液进行的授精在第二天进行了授精，其中检测到阴道前阴道结合黄体酮浓度 10ng / ml使用计算机辅助精液分析（CASA）分析所有精液样品的总精子数，总运动能力和进行性运动能力授精剂量是基于逐步运动的正常精子（PMNS） 如前所述，使用输尿管镜补充精液在精液扩张和补充子宫后接受精液精液和额外的延长剂在15分钟内慢慢授精通过配备7 Hz探针的B模式超声波测定妊娠和/或通过whe ping率测定精子受精的数量范围为9×10 6〜5 5×10 6个PMNS，进行运动值在20％〜25％之间，25％总体怀孕率为68％25（238/352） 当PMNS分层时，妊娠率如下： 150脳10 / sup PMNS - 76％25（110/145）100 脳10 sup 6 / sup - 68％25（87/128）和100脳10 sup 6 / sup PMNS - 52％25（41/79） 妊娠率显着高于 150脳10 sup 6 / sup PMNS（p \x3d 003）或100 脳10 sup 6 / sup PMNS（p \x3d ）与100脳10 sup 6 / sup PMNS were这些数据与以前的报告一致，建议授予 150脳10 / sup PMNS以最大限度地提高妊娠率这些结果表明，一次最佳时机的EIU受精导致与以前的出版物相似的怀孕率，或使用挪威导管的两个或多个非最佳定时授精
SJ Mason[*]. A retrospective clinical study of endoscopic-assisted transcervical insemination in the bitch with frozen–thawed dog semen[J]. Reproduction in Domestic Animals, 2017,52(Sup2): 275-280