摘要
Two experiments were conducted to evaluate the new rooster semen freezing extender which is containing a low level of glycerol and soybean lecithin as an alternative protective agent in the extender. The aim of the first experiment was to evaluate a new extender for freeze-thawing rooster semen known as “Nabi” extender compared to Beltsville. Second experiment was also performed to determine whether the Nabi extender has negative reactions on fertilization after artificial insemination (AI) or no. In the first experiment, post-thaw motion parameters, mitochondrial function and sperm apoptosis were analyzed using Sperm Class Analyzer (SCA), rhodamine-123 and Annexin-V, respectively for frozen-thawed semen in Nabi and Beltsville extender. Results showed that total motility, progressive motility, velocity parameters (VCL, VSL, VAP, LIN and STR) and live spermatozoa with active mitochondria were significantly higher in Nabi compare to Beltsville extender (P < 0.01). Also, the percentages of post-thawed live and early apoptotic spermatozoa were significantly higher in Nabi compared to Beltsville extender (14.46 ± 0.95 vs. 19.27 ± 0.95 and 14.83 ± 4.51 vs. 39.27 ± 4.51, respectively). For apoptotic spermatozoa, the percentages of post-thawed late apoptotic spermatozoa were significantly lower in Nabi (29.66 ± 3.11) compared to Beltsville extender (69.07 ± 3.11), but the type of extender had no effect on the percentages of post-thawed necrotic spermatozoa. In the second experiment, 20 broiler breeder hens (Ross 308) were inseminated with thawed semen using the new freezing diluents or fresh semen for determination of fertility rate. Fertility rate with thawed semen (with Nabi extender) was lower compared to fresh semen (by approximately 8% points). It can be concluded that Nabi extender would improve post-thawed rooster sperm in vitro quality compared to Beltsville extender. The fertility rates of insemination in hens with freeze-thaw sperm were comparable with fresh sperm.
摘要译文
两个实验以评价新公鸡精液冷冻其中含有甘油和大豆卵磷脂的低水平作为替代保护剂中的扩扩第一个实验的目的是评估相比贝尔茨维尔为称为鈥淣ABI鈥增量冻融公鸡精液一个新扩展器还进行了第二个实验，以确定彩蝶延长是否经过人工授精（AI）或诺茵第一个实验施肥负面反应，采用精子分析仪类（SCA），罗丹明123和Annexin-V解冻后的运动参数，线粒体功能和精子凋亡进行了分析，分别为彩蝶和贝尔茨维尔extenderResults冷冻精液解冻后发现，总蠕动，进步运动，速度参数（VCL，VSL，VAP，LIN和STR）和主动线粒体活精子彩蝶比较贝尔茨维尔延长（P 0Also有显著高，后解冻直播和早期凋亡精子的百分比，在彩蝶显著高于较贝尔茨维尔扩展（14卤0vs19卤0和14卤4vs39卤4分别）对细胞凋亡精子的比例后解冻晚期凋亡精子彩蝶有显著降低（29卤3相比，贝尔茨维尔扩展（69卤3，但延长的类型对后解冻坏死spermatozoaIn第二个实验的百分比没有影响，20肉鸡种鸡（罗斯308）使用新的冷冻稀释剂或新鲜精液测定生育率的精液解冻后受精了与解冻精液（与彩蝶延长）生育率较低相比，新鲜精液（约8％，25分）它可以得出结论，纳比增量将相比贝尔茨维尔延长改善后解冻公鸡精子在体外质量人工授精与冻融精子母鸡的生育率是新鲜的精子相媲美
Mohammad Mahdi Nabi[a]; Hamid Kohram[a][b]; Mahdi Zhandi[a]; Hassan Mehrabani-Yeganeh[a]; Hossein Sharideh[a]; Ahmad Zare-Shahaneh[a]; Vahid Esmaili[c]. Comparative evaluation of Nabi and Beltsville extenders for cryopreservation of rooster semen[J]. Cryobiology, 2016,72(1): 47–52