期刊文献

Comparison of commercial Triladyl extender with a tris-fructose-egg-yolk extender on the quality of frozen semen and pregnancy rate after transcervical AI in Bangladeshi indigenous sheep (Ovis aries) 收藏

商业三聚体补充剂与三 - 果糖 - 蛋黄补充剂对孟加拉国土着绵羊（Ovis aries）中子宫颈AI后的冷冻精液质量和妊娠率的比较。
原文求助 发布源
作者
Azizunnesa Rekha[a]; Begum Fatema Zohara[b]; FaridaYeasmin Bari[c]; Md Golam Shahi Alam[c]
作者单位
[a] Department of Medicine and Surgery, Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong 4202, Bangladesh [b] Department of Medicine, Surgery and Obstetrics, Faculty of Veterinary & Animal Science, Hajee Mohammad Danesh Science & Technology University, Basherhat, Dinajpur 5200, Bangladesh [c] Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh Received 25 May 2015, Revised 10 December 2015, Accepted 12 December 2015, Available online 17 December 2015
关键词
Bangladesh; Indigenous ram; Frozen semen; Semen extenders; TCAI; Pregnancy rate
关键词译文
孟加拉国;土着公羊;冷冻精液;精液补充剂; TCAI;妊娠率
发布日期
17 December 2015
页码
39–43
DOI
10.1016/j.smallrumres.2015.12.007
来源信息
Small Ruminant Research ISSN：0921-4488, 2016年, 134卷, 39–43页
摘要
The objective of this study was to compare the effects of commercial and locally manufactured tris-fructose-egg-yolk semen extenders on the quality of frozen semen and pregnancy rates (PR) after AI of Bangladeshi indigenous sheep. Semen was collected weekly by artificial vagina (AV) from 6 mature indigenous rams (Ovis aries). Each semen sample was diluted with commercial (Triladyl®) and locally manufactured (tris, fructose, egg-yolk, 7% glycerol) extenders, to a final concentration of 200, 400 and 600 × 106 sperm/mL. Semen samples were frozen in 0.25 mL mini-straws in liquid nitrogen (LN) vapor for 6 min and then stored in LN. Post-thawing motility, viability, functional integrity and normal morphology were evaluated on Days 1, 5 and 30 of storage. Post-thaw sperm quality parameters were better (p < 0.001) in Triladyl than in the locally manufactured extender; and quality parameters were also higher for a pre-freezing concentration of 400 × 106 sperm/mL (p < 0.001) than for other concentrations, regardless of extender. Storage time did not affect semen quality parameters (p > 0.001). Indigenous ewes (n = 46) were inseminated transcervically using semen from both extenders at a sperm concentration of 400 × 106/mL. There was no difference in the pregnancy rates between sperm frozen in Triladyl compared with the locally manufactured extender (p > 0.70). In conclusion, whilst post-thaw sperm quality was better in semen frozen in Triladyl, this did not result in any significant benefit compared with the locally-prepared extender on PR after TCAI in ewes.
摘要译文
以比较商业和本地制造的三果糖 - 蛋黄精液补充剂对孟加拉国土着绵羊的AI后的冷冻精液和妊娠率（PR）的质量的影响每周通过人工阴道（AV）从6个成熟的本地公羊（Ovis aries）收集精液。每个精液样品用商业（Triladyl）稀释，并且本地制造（tris，果糖，蛋黄，7％25甘油）补充剂，至终浓度为200,400和600μg/ mL的精子/mL Semen样品在0℃下冷冻25 mL微型吸管在液氮（LN）蒸气中6分钟，然后储存在LN中。在第1天，第5天和第10天评价解冻能动性，活力，功能完整性和正常形态。5和30的存储解冻精子质量参数在三甲苯二胺中比在本地制造的补充剂中更好（p 0 ）;和质量参数对于预冷冻浓度为400μg/ mL的精子/ mL（p 0 ）也高于其他浓度，而不考虑增量剂储存时间不影响精液质量参数（p0 ） 使用来自两种补充剂的精子在精子浓度为400×10 6 /μL/ mL的情况下，用子宫颈授精对土着母羊（n \x3d 46）在三氯杀螨醇冷冻的精子与本地制造的补充剂之间的妊娠率没有差异（p 0）而在Triladyl中冷冻的精液中的融化后精子质量更好，与在远东地区的TCAI后PR上的局部制备的延长剂相比，没有产生任何显着的益处
Azizunnesa Rekha[a]; Begum Fatema Zohara[b]; FaridaYeasmin Bari[c]; Md Golam Shahi Alam[c]. Comparison of commercial Triladyl extender with a tris-fructose-egg-yolk extender on the quality of frozen semen and pregnancy rate after transcervical AI in Bangladeshi indigenous sheep (Ovis aries)[J]. Small Ruminant Research, 2016,134: 39–43