摘要
In whole-body-irradiated (WBI) mice, levels of the canonical Th1 cytokine IFN-gamma (IFNG) have been shown to be markedly reduced, resulting in a Th1/Th2 imbalance. In this study, the influence of natural killer (NK) cells on the balance of this Th1/Th2 immune response was evaluated in WBI mice. Although NK cells are one of the types of cells that secreteIFN-gamma, NK cell activity tends to be minimal, even at 7 weeks after irradiation. In NK cell-depleted mice, the levels of Th1-related cytokines were lower than those of the control mice and were correlated with lower IgG2a production and elevated IgE and IgG1 production. These results indicated that NK cells have a crucial role in the final differentiation of Th cells into Th1 cells. The impairment of NK cells in the WBI mice was confirmed by the observation that NK cells from the WBI mice induced a decrease in the generation of IFN-gamma by the NK cell-depleted spleen lymphocytes from normal mice. Also, the WBI mice that received NK cells obtained from the normal mice generated more IgG2a, IL12 and IFN-gamma. Our results indicate that the impairment of NK cells is an important factor in the reduced Th1-like response in irradiated mice.
摘要译文
在全身照射（WBI）小鼠中，规范的Th1细胞因子IFN-γ（IFNG）的水平已被证明是显着降低，导致Th1 / Th2失衡。在这项研究中，自然杀伤（NK）细胞上的这种Th1 / Th2型免疫应答的平衡的影响在WBI小鼠进行评价。虽然NK细胞是secreteIFN-γ的类型之一，NK细胞活性的倾向是最小的，即使是在7周照射后。在NK细胞耗尽的小鼠中，的Th1相关的细胞因子的水平高于对照小鼠的低，均与较低的IgG2a生产和升高的IgE和IgG1的生产。这些结果表明，NK细胞具有在Th细胞的最终分化成Th1细胞至关重要的作用。NK细胞的小鼠的WBI通过从WBI小鼠NK细胞诱导的IFN-γ的产生减少由从正常小鼠的NK细胞耗尽的脾淋巴细胞的观察证实。同样，接收到来自正常小鼠获得NK细胞的WBI小鼠中产生更多的IgG2a，IL12和IFN-γ。我们的研究结果表明，NK细胞的损伤是在辐射损伤小鼠的降低的Th1样反应的一个重要因素。
Park HR; Jung U; Jo SK. Impairment of natural killer (NK) cells is an important factor in a weak Th1-like response in irradiated mice.[J]. Radiation Research, 2007,168(4): 446-452