摘要
Four adult Simmental male cattle (376 ± 9.0 kg initial BW), fitted with permanent rumen cannulas, were used in a 4 × 4 Latin square design to investigate the effects of dietary supplementing tannic acid (TA) on rumen fermentation, methane (CH4) production, rumen microbes, nutrient digestibility and plasma biochemical parameters. Four levels of TA, that is 0, 6.5, 13.0 or 26.0 g/kg dry matter (DM), were added to the basal ration (composed of corn silage and concentrate mixture) as experimental treatments respectively. Each experimental period consisted of a 12-day adaptation phase followed by a 3-day sampling phase. The results showed that supplementing TA at 26.0 g/kg DM decreased the relative abundance of protozoa, methanogens and Ruminococcus albus to the total ruminal bacterial 16S rDNA in beef cattle (p < 0.05). The results also showed that supplementing TA at 6.5, 13.0 or 26.0 g/kg DM decreased (p < 0.01) the CH4 production (l/kg DM intake) by 11.1%, 14.7% and 33.6% respectively. Supplementing TA at 13.0 or 26.0 g/kg DM decreased the ratio of acetate to propionate and ammonia nitrogen (NH3–N) (p < 0.05) and tended to decrease the total volatile fatty acid (VFA) concentration of rumen fluid (p = 0.07). Supplementing TA at 26.0 g/kg DM decreased DM and organic matter (OM) digestibility (p < 0.05), supplementing TA at 6.5, 13.0 or 26.0 g/kg DM decreased (p < 0.01) crude protein (CP) digestibility by 5.0%, 8.6% and 15.7%, respectively, and supplementing TA at 6.5, 13.0 or 26.0 g/kg DM increased (p < 0.05) the plasma total antioxidant capability. It was concluded that supplementing TA in the ration of beef cattle decreased the CH4 production and digestibility of CP of beef cattle. Supplementing TA could be an effective option to mitigate CH4 emission form cattle, further research is necessary to study the effects of TA on the performance of cattle.
摘要译文
四只成年西门塔尔男牛（376±9 初始BW），配有永久性瘤胃插管，用于4×4拉丁方格设计，以研究膳食补充单宁酸（TA）对瘤胃发酵，甲烷（CH 4）产生，瘤胃微生物，营养消化率和血浆生化指标。TA的水平，即0,6 13 r26 / kg干物质（DM），分别添加到玉米青贮饲料和浓缩混合物的基础配比中作为实验处理每个实验期包括12天适应阶段，随后是3天取样阶段。结果表明，补充TA在26 0 g / kg DM将原生动物，产甲烷菌和粗糙球菌的相对丰度降低到肉牛总瘤胃细菌16S rDNA（p 0 结果还显示，补充6月13日26日/ kg DM的TA降低（p 0，CH 4产量（l / kg DM摄入）分别为11 5,14.55和33 5。补充TA在13 0或26 / kg DM降低了乙酸盐与丙酸盐和氨氮（NH 3 - ）的比例（p 0），倾向于降低瘤胃总挥发性脂肪酸（VFA）浓度（p \x3d0 07） 26 / kg DM补充DM DM DM DM DM DM DM DM DM DM DM DM（（（（（（（（（（（（（（（（（（（（（（（（（（（（（（（01）粗蛋白（CP）消化率分别为5 5,8 5和15 5，补充TA在6 13 26 / kg DM下增加（p 0 血浆总抗氧化能力得出结论，在肉牛配给中补充TA降低了肉牛的CH 4产量和CP消化率补充TA可能是减轻牛的CH 4排放的有效选择，需要进一步的研究来研究TA对牛的表现的影响
K. Yang, C. Wei, G. Y. Zhao[*]; Z. W. Xu and S. X. Lin;. Effects of dietary supplementing tannic acid in the ration of beef cattle on rumen fermentation, methane emission, microbial flora and nutrient digestibility[J]. Journal of Animal Physiology and Animal Nutrition, 2017,101(2): 302-310