摘要
A collection of Acinetobacter strains consisting of 168 consecutive clinical strains and 30 type and reference strains was studied by DNA-DNA hybridization and a few phenotypic tests. The field strains could be allotted to 13 DNA groups. By means of reference strains ten of these could be identified with groups described by Bouvet & Grimont (1986), while three groups were new; they were given the numbers 13-15. The type strain of A. radioresistens- recently described by Nishimura et al. (1988) - was shown to be a member of DNA group 12, which comprised 31 clinical isolates. Of the 19 strains of A. junii, eight showed hemolytic activity on sheep and human blood agar and an additional four strains on human blood agar only. Strains of this species have previously been regarded as non-hemolytic. Reciprocal DNA pairing data for the reference strains of the DNA groups were treated by UPGMA clustering. The reference strains for A. calcoaceticus, A. baumannii and for DNA groups 3 and 13 formed a cluster with about 70% relatedness within the cluster. Other DNA groups joined at levels below 60%. .overlined { text-decoration: overline; } .struck { text-decoration:line-through; } .underlined { text-decoration:underline; } .doubleUnderlined { text-decoration:underline;border-bottom:1px solid #000; } Get PDF (700K)Get PDF (700K) More content like thisFind more content: like this articleFind more content written by: INGELA TJERNBERG JAN URSING All Authors
摘要译文
通过DNA-DNA杂交和一些表型试验来研究由168个连续临床菌株和30个类型和参考菌株组成的不动杆菌菌株的集合。野外菌株可分配到13个DNA组。通过参考菌株,其中10个可以用Bouvet&Grimont(1986)描述的组鉴定,而三个组是新的;他们被给了13-15号码。最近由Nishimura等人描述的A. radioresistens的类型应变(1988) - 被证明是DNA组12的成员,其中包括31个临床分离株。在19株菌株中,8株在绵羊和人血琼脂上表现出溶血活性,另外4种在人血琼脂上表现出溶血活性。此种菌株以前被认为是非溶血性的。通过UPGMA聚类处理DNA组的参考菌株的互补DNA配对数据。醋酸钙不动杆菌,鲍曼不动杆菌和DNA组3和13的参考菌株形成簇内具有约70%相关性的簇。其他DNA基团以低于60%的水平连接。 。[overlined {text-decoration:overline; } .struck {text-decoration:line-through; } .underlined {text-decoration:underline; } .doubleUnderlined {text-decoration:underline; border-bottom:1px solid#000;]}获取PDF(700K)获取PDF(700K)更多类似这样的内容查找更多内容:像这篇文章查找更多内容撰写者:英吉拉·泰伯格·詹·乌斯林所有作者
INGELA TJERNBERG and JAN URSING[*]. Clinical strains of Acinetobacter classified by DNA-DNA hybridization[J]. APMIS, 1989,97(7-12): 595-605