摘要
The major histocompatibility complex (MHC) is one of the most important elements in immune system for nearly all vertebrates, and is thought to be essential for an organism to recognize foreign molecules. In this study, we investigated the MHC variation in 51 forest musk deer (Moschus berezovskii) collected from three captive populations in Sichuan Province, China. Seventeen haplotypes were isolated from the 51 samples. A total of 51 mutation sites were identified and yielded a nucleotide diversity of 0.056. These haplotype sequences possessed 83 putative amino acid sites, including 24 PBR sites (peptide binding region) and 59 non-PBR sites. Out of 24 PBR sites, 15 codons showed variation (0.625), while 12 codons showed variation (0.203) in 59 non-PBR sites. Non-synonymous substitutions primarily occurred in PBR, with analyses suggesting that the Mobe-DRB gene had undergone strong positive selection during their evolution. Compared with that of some other endangered species, the forest musk deer had relatively high level of MHC diversity. Our results suggested that the MHC diversity characteristic of captive forest musk deer populations might be not responsible for their high morbidity of abscess disease.
摘要译文
主要组织相容性复合体（MHC）是几乎所有脊椎动物免疫系统中最重要的元素之一，并被认为是生物体识别外源分子的关键。在这项研究中，我们调查了从四川省三个圈养人群收集的51株森林麝香鹿（Moschus berezovskii）的MHC变异从51个样品中分离出十七个单倍型。鉴定了总共51个突变位点并产生了0核苷酸多样性这些单倍型序列具有83个推定的氨基酸位点，包括24个PBR位点的24个PBR位点（肽结合区）和59个非PBR位点 Out，15个密码子显示变异（0 ）而12个密码子在59个非PBR位点显示变异（0 ）。非同义取代主要发生在PBR中，分析表明Mobe-DRB基因在其进化过程中经历了强阳性选择。与其他一些濒危物种相比，森林麝香鹿的MHC多样性水平相对较高我们的研究结果表明，俘虏森林麝香鹿群体的MHC多样性特征可能不负责他们的脓肿疾病的高发病率
Shan Xia[a][b]; Zhenxin Fan[a]; Xiuyue Zhang[a]; Chen Jie[b]; Xiaojuan Zhang[b]; Bisong Yue[c]. Molecular polymorphism of MHC-DRB gene and genetic diversity analysis of captive forest musk deer (Moschus berezovskii)[J]. Biochemical Systematics and Ecology, 2016,67: 37–43