摘要
Bovine cumulus oocyte complexes (COCs) as used for in vitro maturation and fertilization can be classified into different categories by light microscopical inspection. We have distinguished four categories based on compactness and transparency of the cumulus investment and homogeneity and transparency of the ooplasm. The four categories were studied for their morphological characteristics at the ultrastructural level and for their developing capacity in an in vitro maturation system. In categories 1 and 2 oocytes, organelles were evenly distributed. In categories 3 and 4, oocytes organelles were clustered and the distribution of the organelles mimicked the characteristics of oocytes during final maturation. Cumulus cell process endings penetrated the cortex of the oocyte or were located superficial to the cortex of the oocyte. In category 1 oocytes, most of the process endings penetrated the cortex. In category 4 oocytes, most of the process endings did not penetrate. In categories 2 and 3 oocytes, both forms of process endings did occur.
After in vitro maturation, only category 4 oocytes showed a decreased developing capacity. Categories 1–3 oocytes showed equal developing capacity in an in vitro maturation system. .overlined { text-decoration: overline; } .struck { text-decoration:line-through; } .underlined { text-decoration:underline; } .doubleUnderlined { text-decoration:underline;border-bottom:1px solid #000; } Enhanced Article (HTML) Get PDF (775K)Get PDF (775K) More content like thisFind more content: like this articleFind more content written by: F. de Loos C. van Vliet P. van Maurik Th. A. M. Kruip All Authors
摘要译文
用于体外成熟和受精的牛卵母细胞复合体（COCs）可以通过光学显微镜检查分类为不同类别我们基于积云投资的密集度和透明度以及卵胞浆的同质性和透明度来区分四个类别研究了四个类别在超微结构水平的形态特征以及它们在体外成熟系统中的发育能力。在1和2类卵母细胞中，细胞器均匀分布。在类别3和4中，卵母细胞器聚集并且细胞器的分布模拟最终成熟期期间卵母细胞的特征积累细胞过程结束渗透卵母细胞的皮层或位于卵母细胞皮层的表面。在1类卵母细胞中，大多数过程结束渗透皮层在类别4卵母细胞中，大多数过程结束没有渗透到类别2和3卵母细胞，两种形式的过程结束确实发生。在体外成熟后，只有4类卵母细胞显示出降低的发育能力。类型1 - 细胞在体外成熟系统中表现出相等的发育能力。 } struck {text-decoration：line-through; }竨nderlined {text-decoration：underline; }竏oubleUnderlined {text-decoration：underline; border-bottom：1px solid＃000;}增强的文章（HTML）获取PDF（775K）获取PDF（775K）更多内容喜欢这篇文章更多内容：喜欢这篇文章更多内容基于：F deLoosC vanVlietP vanMaurikTh A M 蠕变所有的著者
Dr. F. de Loos[1,*]; C. van Vliet[1]; P. van Maurik[2] and Th. A. M. Kruip[1];. Morphology of immature bovine oocytes[J]. Molecular Reproduction and Development, 1989,24(2): 197-204