摘要
A 30-kDa host factor (polyhedrin-promoter-binding protein; PPBP) specifically binds to sequences critical for transcription from the baculovirus polyhedrin (p29) gene initiator promoter [Burma, S., Mukherjee, B., Jain, A., Habib, S. & Hasnain, S. E. (1994) J. Biol. Chem. 269, 2750–2757; Mukherjee, B., Burma, S. & Hasnain, S. E. (1995) J. Biol. Chem. 270, 4405–4411]. A host factor also binds, in gel shift assays, to the very-late p10 gene promoter through DNA sequence motifs similar to the PPBP p29 interaction. The p10 host factor complex was specifically competed out with oligonucleotides containing p29 cognate sequence motifs AATAAA and TAAGTATT, but this did not occur when these motifs were replaced with random sequences. From ultraviolet cross-linking analysis, the molecular mass of this host factor was estimated to be approximately 30 kDa. Experiments were performed to investigate if this host factor displayed any differences in affinity and turnover with respect to the p29 and p10 untranslated leader sequences known to be important for temporal fine tuning and the late burst of transcription. Half-life determination of the p10-binding protein revealed similar binding affinities for the initiator elements of both the promoters, but higher affinity for the p10 5′-untranslated region (≈30 min versus ≈10 min). The involvement of a similar host factor binding to both the p10 and p29 promoters indicates the possibility of a similar mode of transcription initiation from these two very-late promoters. .overlined { text-decoration: overline; } .struck { text-decoration:line-through; } .underlined { text-decoration:underline; } .doubleUnderlined { text-decoration:underline;border-bottom:1px solid #000; } View Full Article (HTML) Enhanced Article (HTML) Get PDF (839K)Get PDF (839K) More content like thisFind more content: like this articleFind more content written by: Anjali Jain Seyed E. Hasnain All Authors
摘要译文
一个30-kDa的宿主因子（多角体蛋白 - 启动子结合蛋白; PPBP）特异性结合到杆状病毒多角体蛋白（p29）基因启动子启动子转录的关键序列[Burma，Mukherjee，B.，Jain，A.，Habib，S.＆Hasnain，S.E。（1994）J.Biol.Chem。化学。 269，2750-2757; Mukherjee，B。，Burma，S.＆Hasnain，S.E。（1995）J.Biol.Chem。化学。 270，4405-4411]。宿主因子也绑定，在凝胶移位分析中，通过与PPBP p29相互作用类似的DNA序列基序，连接至非常晚的p10基因启动子。p10宿主因子复合物与含有p29同源序列基序AATAAA和TAAGTATT的寡核苷酸特异性竞争，但是当这些图案被随机序列取代时，这不会发生。从紫外线交联分析中，该宿主因子的分子量估计为约30kDa。对于已知对于时间微调和转录后期爆发而言重要的p29和p10非翻译前导序列，亲和力和周转率显示任何差异。p10结合蛋白的半衰期测定显示对于两种启动子的引发剂元件具有类似的结合亲和力，但对p10 5''''-非翻译区的亲和力更高（≈30分钟对比≈10分钟）。与p10和p29启动子结合的相似宿主因子的参与表明从这两个非常晚期的启动子可能有类似的转录启动模式。 。[overlined {text-decoration：overline; } .struck {text-decoration：line-through; } .underlined {text-decoration：underline; } .doubleUnderlined {text-decoration：underline; border-bottom：1px solid＃000;]}查看全文（HTML）增强型文章获取PDF（839K）获取PDF（839K）更多类似的内容查找更多内容：像这篇文章查看更多内容撰写者：Anjali Jain Seyed E.Hasnain所有的作者
Anjali Jain and Seyed E. Hasnain[*]. A 30-kDa Host Protein Binds to Two Very-Late Baculovirus Promoters[J]. FEBS Journal, 1996,239(2): 384-390