摘要
The aim of this study was to evaluate the fertility response of artificial insemination (AI) methods with fresh and frozen sperm in sheep. In experiment 1, one hundred and fifty fat tailed Zandi ewes were assigned into 3 equal groups and inseminated with three AI methods consisting of vaginal, laparoscopic and trans-cervical AI with fresh semen. In experiment 2, a factorial study (3 AI methods × 2 extenders) was used to analyze the effects of three AI methods and two freezing extenders containing soybean lecithin (SL) or Egg yolk (EY) on reproductive performance of 300 fat tailed Zandi ewes. Also, total motility, progressive motility, viability and lipid peroxidation of semen were evaluated after freeze-thawing in two extenders. In result, there was no significant difference among three AI methods when fresh semen was used. In experiment 2, the highest percentage of pregnancy rate, parturition rate and lambing rate were obtained in laparoscopic AI group (P < 0.05). Although pregnancy rate, parturition rate and lambing rate in trans-cervical group were higher (P < 0.05) than vaginal group, the results were not as high as laparoscopic group. No difference was observed between SL and EY extenders and their performance was close to each other. It can be concluded that although no difference was observed on reproductive performance for fresh semen, trans-cervical AI was more efficient than vaginal method when frozen-thawed semen was used, but its efficiency was not as high as laparoscopic method. Also, SL extender can be an efficient alternative extender to preserve ram sperm during cryopreservation procedure without adverse effects of EY.
摘要译文
这项研究的目的是评估人工授精（AI）方法与新鲜和冷冻精子在羊羊中的生育反应在实验1中，将150尾脂肪尾的Zandi母羊分成3个相等的组，并用三种AI方法进行授精，所述AI方法包括具有新鲜精液的阴道，腹腔镜和经宫颈AI在实验2中，脳2延伸剂）用于分析三种AI方法和两种含有大豆卵磷脂（SL）或蛋黄（EY）的冷冻补充剂对300只脂肪尾部Zandi ewes的生殖性能的影响此外，在两种补充剂冻融后，评价精子的总运动性，进行性运动性，活力和脂质过氧化。结果，在使用新鲜精液的三种AI方法之间没有显着差异。在实验2中，妊娠率的最高百分比，分娩率和出血率在腹腔镜AI组中获得（P 0 ，尽管妊娠率，宫颈组的分娩率和羔羊率较高（P 0 ）05）比阴道组高，结果不如腹腔镜组高。在SL和EY延伸剂之间没有观察到差异，并且它们的性能接近彼此可以得出结论，虽然在新鲜精液的生殖性能方面没有观察到差异，但当使用冷冻 - 解冻的精液时，宫颈的AI比阴道方法更有效，但其效率不如腹腔镜法高。此外，SL延长剂可以是一种有效的替代延长剂，在冷冻保存过程中保存公羊精子而没有EY without的不良影响
Reza Masoudi[a]; Ahmad Zare Shahneh[a]; Armin Towhidi[a]; Hamid Kohram[a]; Abbas Akbarisharif[b]; Mohsen Sharafi[c]. Fertility response of artificial insemination methods in sheep with fresh and frozen-thawed semen[J]. Cryobiology, 2017,74: 77–80