图书章节

A Technique to Isolate Sperm Cells of Mature Spinach Pollen 收藏

成熟菠菜花粉精细胞的分离技术
原文求助 发布源
作者
C. H. Theunis1;J. L. van Went1;H. J. Wilms1
作者单位
1.Department of Plant Cytology and MorphologyAgricultural UniversityWageningenThe Netherlands
关键词
Sperm Cell ;Mature Pollen ;Isolation Medium ;Fluorescein Diacetate ;Pollen Wall
关键词译文
精子细胞;成熟花粉;分离培养基;二乙酸荧光素;花粉壁
页码
233-238
DOI
10.1007/978-3-642-73271-3_37
来源信息
Sexual Reproduction in Higher Plants ISBN：9783642732737, 1988年, 233-238页
摘要
A technique has been developed to isolate sperm cells of mature Spinacia oleracea pollen. By using a squashing technique in combination with centrifugation on a percoll layer is it possible to isolate the sperm cells in large amounts, and to keep them viable for several hours.
摘要译文
已经开发出一种技术来分离成熟的Spinacia oleracea花粉的精子细胞。通过将挤压技术与percoll层上的离心结合使用，可以大量分离精子细胞，并使其保持数小时。
C. H. Theunis1;J. L. van Went1;H. J. Wilms1. A Technique to Isolate Sperm Cells of Mature Spinach Pollen. Sexual Reproduction in Higher Plants[M].DE: Springer, 1988: 233-238