摘要
Baculoviruses have been used for many years as effective pest-control agents; however, their interest to molecular virologists stems from their exploitation as helper-independent viral expression vectors for the production of proteins in a eukaryotic environment. The pioneering work that led to the development of the system came from the laboratory of Max Summers at Texas A & M, USA. Since its inception, the system has been increasingly utilized to express a broad range of eukaryotic proteins (reviewed in ref. 1) and has been extensively modified (2), in particular by David Bishop and Bob Possee at the Institute of Virology and Environmental Microbiology, Oxford, UK. This work has led to the construction of single and multiple baculovirus expression vectors. Extensive reviews on the subject of baculoviruses are available (1,3, 4, 5), including a manual of methods specific for their use as expression systems (6). Chapters 26– 28 aim to provide a practical account of baculoviruses as expression vectors, along with the experimental methodologies required to produce a baculovirus transfer vector containing the gene to be expressed, the generation and selection of a recombinant expressing this gene product, and the methods available for processing downstream the baculovirus-produced protein.
摘要译文
杆状病毒作为有效的害虫控制剂已使用多年;然而，他们对分子病毒学家的兴趣源于它们作为辅助非依赖性病毒表达载体的利用，用于在真核环境中产生蛋白质。导致该系统开发的开创性工作来自美国Texas A＆M的Max Summers实验室。自成立以来，该系统已越来越多地用于表达广泛的真核蛋白（参见参考文献）。 1）并且已经进行了广泛的修改（2），特别是英国牛津大学病毒学和环境微生物学研究所的David Bishop和Bob Possee。这项工作导致了单个和多个杆状病毒表达载体的构建。有关杆状病毒主题的广泛评论（1,3,4,5），包括特定用于表达系统的方法手册（6）。第26-28章旨在提供杆状病毒作为表达载体的实际说明，以及产生含有待表达基因的杆状病毒转移载体所需的实验方法，表达该基因产物的重组体的产生和选择，以及方法可用于处理杆状病毒产生的蛋白质的下游。
Vincent C. Emery[1]. Baculovirus Expression Vectors. Practical Molecular Virology[M].DE: Springer, 1992: 287-307