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The Baculovirus-Infected Insect Cell as a Eukaryotic Gene Expression System 收藏

杆状病毒感染的昆虫细胞作为真核基因表达系统

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作者

M. J. Fraser[1]

作者单位

[1]Department of Biological SciencesUniversity of Notre DameNotre DameUSA

关键词

Insect Cell ;Nuclear Polyhedrosis Virus ;Rift Valley Fever Virus ;Baculovirus Expression System ;Baculovirus Vector

关键词译文

昆虫细胞;核多角体病毒;裂谷热病毒;杆状病毒表达系统;杆状病毒载体

页码

131-172

DOI

10.1007/978-3-642-75608-5_6

来源信息

Viral Expression Vectors ISBN：9783642756108, 1992年, 131-172页

摘要

Baculovirus-infected insect cell cultures are established as an easily manipulated eukaryotic system for highly efficient expression of gene products. The system takes advantage of several unique attributes of this virus group, including highly active late gene promoters, the capacity for insertion of large fragments of foreign DNA, replication competence of the resulting recombinants, and the relative ease of handling both the insect cell cultures and the viruses.

摘要译文

杆状病毒感染的昆虫细胞培养物被建立为易于操作的真核系统，用于高效表达基因产物。该系统利用了该病毒组的几个独特属性，包括高活性晚期基因启动子，插入大片外源DNA的能力，所得重组体的复制能力，以及相对容易处理昆虫细胞培养物和病毒。

M. J. Fraser[1]. The Baculovirus-Infected Insect Cell as a Eukaryotic Gene Expression System. Viral Expression Vectors[M].DE: Springer, 1992: 131-172