摘要
Levels of plasma HDL are determined by a complex array of metabolic processes involving synthesis, transfer, recycling, and tissue uptake of individual apoprotein and lipid components1. At equilibrium, these components are organized within a variety of particulate forms, and detailed understanding of HDL metabolism requires consideration of factors responsible for this macromolecular heterogeneity. The first description of the HDL particle spectrum identified three major forms of HDL based on flotation rates (F°1.20) in the analytic ultracentrifuge2: HDL1, of F°1.20>9 and density overlapping with LDL; HDL2, of F°1.20 3.5–9 (d 1.063–1.125 g/ml), and HDL3, of F°1.200–3.5 (d 1.125–1.200 g/ml). Figure 1 shows the analytic ultracentrifuge pattern of HDL2 and HDL3 in a representative normal subject, as well as the HDL profile as revealed by zonal ultracentrifugation3 and electrophoresis of HDL in native polyaerylamide gradient gels4. Each of these procedures has revealed further heterogeneity within the HDL2 and HDL3 density subclasses. In the case of gradient gel electrophoresis, heterogeneity is manifest as multiple electrophoretic bands which have been shown to represent distinct HDL subspecies of differing particle size.
摘要译文
血浆HDL的水平由一系列复杂的代谢过程决定，包括合成，转移，再循环和单个载脂蛋白和脂质成分的组织摄取 1 。在平衡时，这些组分以各种颗粒形式组织，并且对HDL代谢的详细理解需要考虑造成这种大分子异质性的因素。 HDL粒子谱的第一个描述基于分析超速离心机 2 中的浮选速率（F°1.20）确定了三种主要形式的HDL：HDL 1，F°1.20> 9且密度与LDL重叠; HDL 2，F°1.20 3.5-9（d 1.063-1.125g / ml），和HDL 3，F°1.20 0-3.5（d 1.125-1.200g / ml）。图1显示代表性正常受试者中HDL 2和HDL 3的分析超速离心模式，以及通过区域超速离心 3 显示的HDL谱和天然聚丙烯酰胺梯度凝胶中的HDL电泳 4 。这些程序中的每一个都揭示了HDL 2和HDL 3密度亚类内的进一步异质性。在梯度凝胶电泳的情况下，异质性表现为多个电泳条带，其已经显示出代表不同粒度的不同HDL亚种。
Ronald M. Krauss[1];Alex V. Nichols[1]. Metabolic Interrelationships of HDL Subclasses. Lipoprotein Deficiency Syndromes[M].DE: Springer, 1986: 17-27