摘要
This chapter aims to provide the reader with the experimental protocols required to produce a baculovirus containing the gene of interest, including transfection of Spodoptera frugiperda cells and the screening of progeny virus by plaque assay. In addition, an outline of the biochemical methods routinely used to characterize the expressed protein product within the baculovirus system will be given. Throughout the experimental procedures, areas that researchers generally find technically difficult will be highlighted in the hope that this may ameliorate some of the problems encountered in using the system ab initio.
摘要译文
本章旨在为读者提供产生含有目的基因的杆状病毒所需的实验方案，包括转化草地夜蛾（Spodoptera frugiperda）细胞和通过噬斑测定法筛选子代病毒。此外，将给出常规用于表征杆状病毒系统内表达的蛋白质产物的生物化学方法的概述。在整个实验过程中，研究人员通常认为在技术上很困难的领域将会突出显示，希望这可以改善从头开始使用系统时遇到的一些问题。
Vincent C. Emery[1]. Baculovirus Expression Vectors. Practical Molecular Virology[M].DE: Springer, 1992: 309-318