摘要
Successful expression of neurotransmitter receptors in heterologous systems in vitro requires that the host cell can synthesise adequate amounts of correctly processed and targeted protein. To date the most popular expression systems for receptors have been the injection of DNA or mRNA into Xenopus oocytes,1,2 or the use of vectors to transform Escherichia coli,3 yeast4 or mammalian cells, e. g. fibroblasts.5,6 One of the main disadvantages of these systems is that often only very small quantities of protein are synthesised, and in the case of E. coli, the proteins are often not correctly processed to allow functional analyses.
摘要译文
在体外异源系统中成功表达神经递质受体需要宿主细胞可以合成足够量的正确加工和靶向的蛋白质。迄今为止，最受欢迎的受体表达系统是将DNA或mRNA注入非洲爪蟾卵母细胞， 1,2 或使用载体转化大肠杆菌， 3 酵母 4 或哺乳动物细胞，例如G。成纤维细胞。 5,6 这些系统的主要缺点之一是通常只合成非常少量的蛋白质，而在大肠杆菌的情况下，蛋白质通常不能正确加工以允许功能分析。
Linda A. King[1];Allan E. Atkinson[1];Louis A. Obosi[1];Isabel Bermudez[1];David J. Beadle[1]. Expression of Neurotransmitter Receptors in Insect Cells Using Baculovirus Vectors. Neurotox ’91[M].DE: Springer, 1992: 191-202