摘要
The development of expression vectors from baculoviruses has provided molecular biologists with one of the most efficient and effective ways of synthesizing foreign gene products in a heterologous system. In contrast to the other recent excellent reviews on the subject (1,2), this contribution aims to explore the various practical aspects of the system, and in consequence, to highlight particular difficulties that may be encountered by workers new to the field. However, before describing the methodology peculiar to baculovirus expression vectors, it is of value to briefly consider the isolation, structure, and replication strategy of this unique group of viruses. Detailed and more comprehensive reviews that describe the biology of baculoviruses are available (3, 4, 5)
摘要译文
来自杆状病毒的表达载体的开发为分子生物学家提供了在异源系统中合成外源基因产物的最有效和最有效的方法之一。与最近对该主题的其他最佳评论（1,2）相比，该贡献旨在探索该系统的各种实际方面，并因此突出了该领域新工作者可能遇到的特殊困难。然而，在描述杆状病毒表达载体特有的方法之前，简要地考虑这种独特病毒组的分离，结构和复制策略是有价值的。可以获得描述杆状病毒生物学的详细和更全面的评论（3,4,5）
Murk J. Bailey[1];Robert D. Possee[1]. Manipulation of Baculovirus Vectors. Gene Transfer and Expression Protocols[M].DE: Springer, 1991: 147-168