摘要
This study was conducted to investigate the effects on fermentation characteristics of rumen microorganism by different types and levels of lignosulfonate treated soybean meal (LSBM) in in vitro test and rumen simulation continuous culture (RSCC) system in dairy cows. The experiment I was control and 12 treatments (each with 3 replications) in vitro test to demonstrate composition of different types of treatments with lignosulfonate (Desulfonate, Na, Ca and solution) and levels (2, 4 and 8%) of soybean meal in the dairy cow diet. LSBM source treatments in the dairy cow diet showed pH value, NH3 -N concentration and total VFA concentration lower than control at all levels and incubation times (p<0.05). Dry matter digestibility of LSBM source treatments showed lower than control (p<0.05). Gas production and rumen microbial synthesis was decreased by rumen microbial fermentation for incubation times. Undegradable protein (UDP) concentration of all LSBM treatments was decreased for incubation times, and significantly higher than control (p<0.05). In the experiment II compared diets of the control, LSBM Na 2%, LSBM Sol 2%, which are high performance to undegradable protein (UDP) concentration experiment I in vitro test, and heated treatment lignosulfonate (LSBM Heat) 2% in the dairy cow diet from four station RSCC system (4×4 4{\times}4 Latin square). A rumen microbial fermentation characteristic was stability during 12~15 days of experimental period in all treatments. The pH value of LSBM treatments was higher than control treatment (p<0.05). The NH3 id="MathJax-Element-3" NH_3 -N concentration, VFA concentration and rumen microbial synthesis of LSBM treatments were lower than control (p<0.05). The undegradable protein (UDP) showed LSBM Na 2% (45.28%), LSBM Sol 2% (43.52%) and LSBM Heat 2% (43.49%) higher than control (41.55%), respectively (p<0.05). Those experiments were designed to improve by-pass protein of diet and milk protein in the dairy cows. We will conduct those experiments the in vivo test by LSBM treatments in dairy cows diet.
摘要译文
本研究旨在研究不同类型和水平的木质素磺酸盐处理过的豆粕（LSBM）和瘤胃模拟连续培养（RSCC）系统对瘤胃微生物发酵特性的影响。本实验为对照，采用12种处理方法（每组重复3次）进行体外试验，以证明木质素磺酸盐（脱磺酸盐，Na，Ca和溶液）不同处理类型的组成以及豆粕中大豆粉的水平（2％，4％和8％）。奶牛饮食。在所有水平和孵育时间下，奶牛日粮中的LSBM来源处理均显示pH值，NH3-N浓度和VFA总浓度均低于对照组（p <0.05）。 LSBM源处理的干物质消化率低于对照（p <0.05）。瘤胃微生物发酵可降低产气量和瘤胃微生物合成时间。所有LSBM处理的不可降解蛋白（UDP）浓度随着培养时间的延长而降低，并且显着高于对照（p <0.05）。在实验II中，比较了对照组饮食中的LSBM Na 2％，LSBM Sol 2％对不降解蛋白质（UDP）浓度较高的实验I，在体外实验中，对乳制品中木质素磺酸盐（LSBM Heat）进行了加热处理2％四站RSCC系统（4×4 4 {—次} 4拉丁方）的奶牛日粮。在所有处理中，瘤胃微生物发酵特性在实验期间的12〜15天都是稳定的。 LSBM处理的pH值高于对照处理（p <0.05）。 LSBM处理的NH3 id = MathJax-Element-3 NH_3-N浓度，VFA浓度和瘤胃微生物合成均低于对照（p <0.05）。不可降解蛋白（UDP）的LSBM Na 2％（45.28％），LSBM Sol 2％（43.52％）和LSBM Heat 2％（43.49％）分别高于对照（41.55％）（p <0.05）。这些实验旨在改善奶牛的饮食蛋白和牛奶蛋白。我们将通过LSBM处理奶牛饮食进行体内实验。
Lee; Hun-Jong;Lee; Seung-Heon;Bae; Gui-Seck;Park; Je-Hwan;Chang; Moon-Baek;. Effects on the Rumen Microbial Fermentation Characteristics of Lignosulfonate Treated Soybean Meal[J]. Journal of Animal Science and Technology, 2010,52(5): 413-426