摘要
Sperm-zona pellucida (ZP) binding is the initial step in fertilization. In this study, to examine sperm-ZP binding properties, we collected ZPs from oocytes obtained from gilt ovaries after 0, 20, 44, and 68 h of maturation culture, and counted the number of sperm bound to the ZP (NSBZ) after 2 min of sperm-ZP co-incubation. Culture of cumulus oocyte complexes (COCs) for 44 h produced significantly higher NSBZ than the other culture durations. Culture of denuded oocytes (DO) produced the same result. To examine the effect of cumulus cells (CCs) and the maturity of oocytes on the NSBZ after maturation culture for 44 h, three oocyte culture conditions (COCs, DO, and combination of DO and CCs) were established, and oocytes were categorized according to the presence of a polar body (PB). The NSBZ did not differ among the culture conditions. NSBZ of oocytes with a PB was greater than that of oocytes without a PB. The addition of tunicamycin, a potent inhibitor of N-linked glycosylation, to the COC maturation medium significantly decreased the NSBZ, although the maturation rate was not affected. In conclusion, NSBZ increases during oocyte maturation and oocytes play a key role in increasing the NSBZ via modification of N-linked glycosylation.
摘要译文
精子-透明带（ZP）结合是受精的第一步。在这项研究中，为了检查精子与ZP的结合特性，我们从成熟培养0、20、44和68小时后的后备卵巢中获得的卵母细胞中收集了ZP，并计算了2次后与ZP（NSBZ）结合的精子数量精子与ZP共同孵育的分钟数。卵丘卵母细胞复合物（COCs）培养44小时产生的NSBZ明显高于其他培养时间。剥脱卵母细胞（DO）的培养产生了相同的结果。为了研究卵丘细胞（CCs）的作用和卵母细胞的成熟对NSBZ成熟培养44小时的影响，建立了三种卵母细胞培养条件（COCs，DO以及DO和CCs的组合），并根据卵母细胞的分类进行了分类。极性体（PB）的存在。 NSBZ在培养条件之间没有差异。有PB的卵母细胞的NSBZ大于没有PB的卵母细胞的NSBZ。尽管不影响成熟速率，向COC成熟培养基中添加了一种有效的N联糖基化抑制剂衣霉素，显着降低了NSBZ。总之，在卵母细胞成熟过程中，NSBZ增加，而卵母细胞通过修饰N-联糖基化在增加NSBZ中起关键作用。
Yosuke Sakaguchi[1];Ryosuke Uzuhashi[1];Hisataka Iwata[1];Yasunori Monji[1];Takehito Kuwayama[1]. Changes in the Sperm-zona Pellucida Binding Properties during Porcine Oocyte Maturation[J]. Journal of Mammalian Ova Research, 2010,27(3): 130-135