摘要
The effect of GnRH administration on superovulatory response of ewes treated with equine chorionic gonadotrophin (eCG) in breeding and nonbreeding seasons and the contribution of laparoscopic insemination to the improvement of fertilization and embryo recovery were investigated. Twenty-four nonpregnant Awassi ewes of 3–4 years of age were randomly allocated into two groups (n = 12). Each ewe was treated with a progesterone impregnated intravaginal sponge for 12 days. The following superovulation treatment was used: ewes of group 1 received 1,200 IU of eCG once as an intramuscular injection 48 h prior to sponge withdrawal; ewes of group 2 also received 1,200 IU of eCG once as an intramuscular injection, 48 h prior to sponge withdrawal and after 24 h of sponge removal. Ewes were injected with 80 μg of GnRH. Ewes of groups 1 and 2 were further subdivided into four equal groups (n = 6). Subgroups A and C (superovulated with eCG and eCG plus GnRH, respectively) were mated naturally at least two times with Awassi rams of proven fertility at 8-h intervals. Subgroups B and D (same as A and C) had intrauterine insemination at 44–46 h after sponge removal, under laparoscopic visualization of uterine horns, depositing 1 ml of diluted semen containing 100 × 106 motile sperm in the distal portion of each uterine horn. Ovarian response was assessed by determining the number of corpora lutea by laparoscopy at day 6 after mating. Embryo recovery was performed by using a semi-laparoscopic flushing procedure in both uterine horns. Results of the present study showed that ewes treated in breeding season with eCG plus GnRH has a higher number (P < 0.05) of corpora lutea than eCG alone as 7.33 ± 0.54 and 4.33 ± 0.39, respectively. There was no significant difference in the number of corpora lutea in nonbreeding season when ewes treated with eCG and eCG plus GnRH. The number of unovulated follicles was significantly higher (P < 0.05) in eCG treated ewes than in ewes treated with eCG plus GnRH, both in the breeding and nonbreeding seasons. The number of recovered embryos from ewes treated with eCG plus GnRH and eCG differ significantly (P < 0.05) as 4.32 ± 0.56 and 1.06 ± 0.26, respectively, in the breeding seasons. No significant difference was observed when these hormones used for superovulation in the nonbreeding season. A higher number of unfertilized ova (P < 0.05) was observed in ewes when naturally inseminated than in ewes inseminated using the intrauterine laparoscopic technique. Higher rate of embryo recovery (P < 0.05) was achieved when ewes were inseminated via intrauterine (4.66 ± 0.66) compared with ewes naturally mated (2.16 ± 0.74). The fertilization rate in ewes inseminated intrauterine using laparoscopic techniques and naturally mated were 91.5% and 44.8%, respectively. Fertilization failure in ewes inseminated intrauterine using laparoscopic techniques and naturally mated were 8.4% and 55.2%, respectively. It could be concluded that administration of GnRH 24 h after sponge removal increased ovulation rate of Awassi ewes treated with eCG for superovulation in the breeding season. The use of eCG to induce superovulation in Awassi ewes combined with laparoscopic intrauterine insemination increases the fertilization rate.
摘要译文
第i个马绒毛膜促性腺激素（心电图）的繁殖和nonbreeding季节和腹腔镜人工授精的受精和胚胎复苏改善的贡献进行了调查。3-4岁二十四个非妊娠Awassi母羊随机分为2组（正每个母羊与浸渍阴道海绵12天孕酮治疗。下面的超数排卵处理后使用：第1组的母羊收到1，200心电图IU曾经作为肌肉注射48小时海绵撤离之前，第2组的母羊也获得了1200 IU心电图一度作为肌肉注射，48小时前撤出海绵和海绵去除后24 h。母羊注射80亚组A和C（超排心电图，心电图以及促性腺激素释放激素，分别）自然交配至少两次的证明生育Awassi公羊在8小时的时间间隔。分组B和D（同A和C）有宫腔内人工授精的海绵取出后44-46小时，在子宫角腹腔镜可视化，沉积1ml含100稀释精液卵巢反应是通过确定黄体的数量腹腔镜在第6天交配后评估。胚胎恢复通过使用在两个子宫角的半腹腔镜冲洗过程进行。本研究的结果表明，母羊在处理繁殖季节心电图以及促性腺激素释放激素有一个较大的数字（P54和4.33unovulated卵泡的数量是显著升高（P恢复胚胎从心电图处理母羊加促性腺激素释放激素及心电图显著不同的数量（P在繁殖季节。未观察到显著的差异时，使用这些激素超排在nonbreeding季节。数字越大，未受精的卵子（P05）观察母羊当自然受精比母羊使用宫内腹腔镜技术授精。率较高胚胎复苏（P05）当母羊通过宫内人工授精分别达到（4.66受精率在母羊子宫内受精采用腹腔镜技术和自然交配率分别为91.5％和44.8％。在母羊受精失败宫腔内人工授精采用腹腔镜技术和自然交配分别为8.4％和55.2％，分别为。它可以得出结论，海绵取出后24小时管理促性腺激素释放激素的增加，心电图治疗超排在繁殖季节Awassi母羊排卵率。利用心电图诱发超排在Awassi母羊联合腹腔镜宫腔内人工授精提高受精率。
Osama Ibrahim Azawi [1] Muzahim Khider Mahmood Ahmed Al-Mola [1]. A study on the effect of GnRH administration on the ovarian response and laparoscopic intrauterine insemination of Awassi ewes treated with eCG to induce superovulation[J]. Tropical Animal Health and Production, 2011,43(7): 1351-1355