摘要
Development of inhibitors and vaccines that mitigate rumen-derived methane by targeting methanogens relies on knowledge of the methanogens present. We investigated the composition of archaeal communities in the rumens of farmed sheep (Ovis aries), cattle (Bos taurus) and red deer (Cervus elaphus) using denaturing gradient gel electrophoresis (DGGE) to generate fingerprints of archaeal 16S rRNA genes. The total archaeal communities were relatively constant across species and diets, and were less variable and less diverse than bacterial communities. There were diet- and ruminant-species-based differences in archaeal community structure, but the same dominant archaea were present in all rumens. These were members of three coherent clades: species related to Methanobrevibacter ruminantium and Methanobrevibacter olleyae; species related to Methanobrevibacter gottschalkii, Methanobrevibacter thaueri and Methanobrevibacter millerae; and species of the genus Methanosphaera. Members of an archaeal group of unknown physiology, designated rumen cluster C (RCC), were also present. RCC-specific DGGE, clone library analysis and quantitative real-time PCR showed that their 16S rRNA gene sequences were very diverse and made up an average of 26.5% of the total archaea. RCC sequences were not readily detected in the DGGE patterns of total archaeal 16S rRNA genes because no single sequence type was abundant enough to form dominant bands.
摘要译文
这减少瘤胃衍生甲烷针对甲烷抑制剂和疫苗的发展依赖于目前的产甲烷菌的知识。这减少瘤胃衍生甲烷针对甲烷抑制剂和疫苗的发展依赖于目前的产甲烷菌的知识。我们研究古群落的组成中养殖的羊瘤胃（绵羊白羊座）我们研究古群落的组成中养殖的羊瘤胃（绵羊白羊座）牛用变性梯度凝胶电泳（DGGE）生成的古16S rRNA基因指纹（普通牛）和红鹿（马鹿）。牛用变性梯度凝胶电泳（DGGE）生成的古16S rRNA基因指纹（普通牛）和红鹿（马鹿）。总的古社区是跨物种和饮食相对恒定，并且是变少，比细菌群落多样性较小。总的古社区是跨物种和饮食相对恒定，并且是变少，比细菌群落多样性较小。有diet-和反刍种系在古细菌群落结构差异，但相同的主古细菌存在于所有的瘤胃。有diet-和反刍种系在古细菌群落结构差异，但相同的主古细菌存在于所有的瘤胃。这些都是三个连贯的分支成员：与甲烷短反刍和甲烷短olleyae种;与甲烷短gottschalkii品种，这些都是三个连贯的分支成员：与甲烷短反刍和甲烷短olleyae种;与甲烷短gottschalkii品种，甲烷短thaueri和甲烷短millerae;和属Methanosphaera的物种。未知生理的古组的成员，指定瘤胃集群C（RCC）甲烷短thaueri和甲烷短millerae;和属Methanosphaera的物种。未知生理的古组的成员，指定瘤胃集群C（RCC）也出席了会议。 RCC-特定DGGE，克隆文库分析和定量实时PCR显示其16S rRNA基因序列是非常多样，由平均26。也出席了会议。 RCC-特定DGGE，克隆文库分析和定量实时PCR显示其16S rRNA基因序列是非常多样，由平均26。5％的总的古。 RCC序列不容易在古共16S rRNA基因的DGGE模式检测，因为没有一个单一序列类型是丰富的足以形成占主导地位的乐队。5％的总的古。 RCC序列不容易在古共16S rRNA基因的DGGE模式检测，因为没有一个单一序列类型是丰富的足以形成占主导地位的乐队。
Jeyamalar Jeyanathan[1,2]; Marek Kirs[1]; Ron S. Ronimus[1]; Simone O. Hoskin[1,2] and Peter H. Janssen[1];. Methanogen community structure in the rumens of farmed sheep, cattle and red deer fed different diets[J]. FEMS Microbiology Ecology, 2011,76(2): 311-326