摘要
In commercial artificial insemination (AI) of sheep, fresh extended semen is deposited into the vagina or cervical os, or fresh extended or frozen semen is placed laparoscopically into the uterus. Transcervical intrauterine insemination of the ewe is not used commercially. In this study, methods of restraint and instrumentation for AI were evaluated and modified to produce a transcervical intrauterine technique suitable for commercial application. Four methods of restraint, four vaginal specula, three forceps and four instruments suitable for transcervical passage were compared. From these comparisons a technique was developed in which the ewes were positioned in dorsal recumbency with their hindquarters elevated. The vagina was dilated using a duck-billed speculum, the cervix was grasped and retracted using forceps, and an inseminating instrument was introduced into the cervical opening and manipulated through the cervical canal. The technique was repeated on 89 mature, multiparous ewes: the difficulty in locating the cervical opening, the force required to retract the cervix and the time required to penetrate into the uterus were recorded. Uterine penetration was achieved in 82% of the ewes. This technique has the potential to be applied in commercial artificial insemination programs of sheep.
摘要译文
在绵羊的商业人工授精（AI）中，新鲜的延长精液沉积在阴道或宫颈口中，或者新鲜的延长或冷冻精液通过腹腔镜置入子宫中。宫颈的子宫内子宫授精不用于商业用途。在这项研究中，对AI的约束和仪器化方法进行了评估和修改，以产生适合商业应用的经宫颈子宫内技术。比较了四种束缚方法，四个阴道镜，三个镊子和四个适合经宫颈通过的器械。通过这些比较，开发了一种将母羊置于背侧卧位并使其后肢抬高的技术。用鸭嘴窥器扩张阴道，用镊子抓住并缩回子宫颈，将授精器械插入子宫颈开口并通过子宫颈管进行操作。在89头成熟的多头母羊身上重复了这项技术：记录了定位子宫颈开口的难度，缩回子宫颈所需的力和穿透子宫的时间。 82％的母羊都有子宫渗透。该技术有可能被用于绵羊的商业人工授精程序。
G.W.Halbert[1][a];H.Dobson[2];J.S.Walton[3];B.C.Buckrell[1];. A technique for transcervical intrauterine insemination of ewes[J]. Theriogenology, 1990,33(5): 993-1010