摘要
The objective of this research was to evaluate the effect of increasing levels of associated glutamine and glutamic acid on growth performance and intestinal development of Nile tilapia, Oreochromis niloticus, fingerlings. Five isoproteic (~344.70 g kg−1 crude protein) and isocaloric diets (~3,925 kcal kg−1 gross energy) were developed containing 0, 5, 10, 15, or 20 g kg−1 of associated glutamine and glutamic acid in extruded diets. Fish (n = 2,000, mean body weight of 2.12±0.53 g) were distributed into twenty 1-m3 floating net cages in an entirely randomized design with five treatments and four replicates, and each replicate comprised one floating net cage with 100 fish. Fish were hand-fed seven days per week, three times a day until apparent satiety for 45 days. There was a quadratic effect on final body weight, body weight gain, daily weight gain, feed conversion ratio, protein retention efficiency, net protein utilization, and intestinal villi height with optimized values for supplementation of associated glutamine and glutamic acid at 10.77, 10.67, 10.00, 8.85, 9.85, 10.15, and 10.98 g kg−1, respectively. There was no effect of associated glutamine and glutamic acid supplementation on feed intake, survival, and body composition. We conclude that 10.67 g kg−1 of associated glutamine and glutamic acid is adequate for growth performance optimization, and supplementation at 10.98 g kg−1 exerts trophic action and improves intestinal morphometry in cage-farmed Nile tilapia fingerlings.
摘要译文
这项研究的目的是评估增加相关的谷氨酰胺和谷氨酸水平对尼罗罗非鱼，尼罗罗非鱼，鱼种的生长性能和肠道发育的影响。开发了五种同质蛋白质（〜344.70 g kg-1粗蛋白）和等热量日粮（〜3,925 kcal kg-1总能量），其中包含0、5、10、15或20 g kg-1的相关谷氨酰胺和谷氨酸经挤压饮食。将鱼（n = 2,000，平均体重为2.12±0.53 g）分配到二十个1立方米的浮式网箱中，采用完全随机设计，进行五次处理和四次重复，每个重复包括一个浮网式网箱，其中有100条鱼。每周7天手工喂鱼，每天3次，直到出现45天的饱腹感。对最终体重，体重增加，日增重，饲料转化率，蛋白质保留效率，净蛋白质利用率和肠绒毛高度有二次效应，其补充谷氨酰胺和谷氨酸的最佳值分别为10.77、10.67，分别为10.00、8.85、9.85、10.15和10.98 g kg-1。补充谷氨酰胺和谷氨酸对饲料的摄入，存活率和身体组成没有影响。我们得出的结论是，相关的谷氨酰胺和谷氨酸10.67 g kg-1足以优化生长性能，而10.98 g kg-1的补充可在笼养农场的尼罗罗非鱼鱼种中发挥营养作用并改善肠道形态。
MACEDO; élison Silva de et al.. Dietary glutamine-glutamate supplementation enhances growth performance and intestinal villi development in cage-farmed Nile tilapia fingerlings.[J]. Revista Brasileira de Zootecnia, 2021,50(50)